Gli-1 Rabbit Polyclonal Antibody (HA500465)
Catalog# HA500465
Rabbit Polyclonal to Gli-1
Tested for
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IHC-P
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IF-Cell
Reacts with
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Human
ICC staining of Gli-1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500465, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).