PRODUCT CODE: ET1702-69

GFP(HRP conjugated) Recombinant Rabbit Monoclonal Antibody [JF51-05] (ET1702-69)

  • Recombinant

Applications

  • WB

REACTIVITY

Western blot analysis of GFP on K562 cells transfected with GFP using anti-GFP antibody at 1/1,000 dilution.
  • Western blot analysis of GFP on K562 cells transfected with GFP using anti-GFP antibody at 1/1,000 dilution.
Western blot analysis of GFP on K562 cells transfected with GFP using anti-GFP antibody at 1/1,000 dilution.

Applications

  • WB

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

GFP(HRP conjugated) Recombinant Rabbit Monoclonal Antibody [JF51-05] (ET1702-69)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562 cells transfected with GFP.

Conjugation

Hrp

Clonality

Monoclonal

Clone Number

JF51-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:10,000-1:20,000

TARGET

PROTEIN NAME

GFP(HRP conjugated)

SYNONYMS

GFP antibody; Green fluorescent protein antibody; yfp antibody

FUNCTION

The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.