GFP Recombinant Rabbit Monoclonal Antibody [SY0243] (ET1607-31)
Catalog# ET1607-31
Rabbit Monoclonal to GFP
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WB
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IF-Cell
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IF-Tissue
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IP
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IHC-P
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Species independent
Lysates/proteins at 50 ng/Lane.
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-31) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Safety datasheet
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- MSDS_HUABIO.pdf
- MSDS_HUABIO.pdf
- MSDS_ET1607-31_Europe.pdf
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COA DOWNLOAD
- No COA Found
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
GFP Recombinant Rabbit Monoclonal Antibody [SY0243] (ET1607-31)
Immunogen
Synthetic peptide within aequorea victoria gfp aa 1-50 / 238.
Host
Rabbit
Positive Control
GFP recombinant protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY0243
RRID
APPLICATION DILUTION
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WB
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1:20,000-1:50,000
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IF-Cell
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1:500-1:1,000
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IF-Tissue
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1:500-1:2,000
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IP
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2-5 µg/ml.
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IHC-P
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1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 27 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GFP
SYNONYMS
GFP antibody;Green fluorescent protein antibody;yfp antibody
FUNCTION
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
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Meng, S., Jagernath, J. S., Luo, C., Shi, H., & Kou, Y.
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Zhou, D., Yan, H., Yang, S., Zhang, Y., Xu, X., Cen, X., Lei, K., & Xia, H.
SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway
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Wang, J., Huang, Z., Huang, P., Wang, Q., Li, Y., Liu, X. H., Lin, F. C., & Lu, J.
The Plant Homeodomain Protein Clp1 Regulates Fungal Development, Virulence, and Autophagy Homeostasis in Magnaporthe oryzae
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Liu Rongrong,et al
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Avian Metapneumovirus Subgroup C Phosphoprotein Suppresses Type I Interferon Production by Blocking Interferon Regulatory Factor 3 Nuclear Translocation
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IF-cell
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Human
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Deng T, Du L, Ding S, et al
Protein kinase Cdc7 supports viral replication by phosphorylating Avibirnavirus VP3 protein
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Human
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Huang, P., Li, Y., Wang, J., Wang, Q., Huang, Z., Liu, X., Lin, F., & Lu, J.
Casein Kinase 2 Mediates Degradation of Transcription Factor Pcf1 during Appressorium Formation in the Rice Blast Fungus
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Gao XK, Sheng ZK, Lu YH, et al
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Mouse
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A Subunit of ESCRT-III, MoIst1, Is Involved in Fungal Development, Pathogenicity, and Autophagy in Magnaporthe oryzae
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Pyricularia Oryzae
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Scophthalmus maximus interleukin-1β limits Edwardsiella piscicida colonization in vivo.
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IF-cell
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Human
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Wu, X., Lai, Y., Rao, S., Lv, L., Ji, M., Han, K., Weng, J., Lu, Y., Peng, J., Lin, L., Wu, G., Chen, J., Yan, F., & Zheng, H.
Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.
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Nicotiana tabacum
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LASS2 regulates hepatocyte steatosis by interacting with NDUFS2/ OXPHOS related proteins
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co-IP
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Tang, H. P., Huang, C., Hu, C. B., Li, H., Shao, T., Ji, J. F., Bai, J., Fan, D. D., Lin, A. F., Xiang, L. X., & Shao, J. Z.
Inhibitory Role of an Aeromonas hydrophila TIR Domain Effector in Antibacterial Immunity by Targeting TLR Signaling Complexes in Zebrafish. Frontiers in microbiology, 12, 694081.
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Zebrafish
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MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae
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MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae
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Zhao Q, He W, Liu Z, et al
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Human
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Zhihuan Tao,et al
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Shunze Jia,et al
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WB
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mice
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MoWhi2 regulates appressorium formation and pathogenicity via the MoTor signalling pathway in Magnaporthe oryzae
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Co-IP
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Rice
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Citation
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Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus
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WB
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Co-IP
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Human
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Suppression of RNA Silencing by a Plant DNA Virus Satellite Requires a Host Calmodulin-Like Protein to Repress RDR6 Expression
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Homeostasis of Arabidopsis R protein RPS2 is negatively regulated by the RING-type E3 ligase MUSE16
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Nicotiana tabacum
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Xie Feiyan,et al
ZBP1 condensate formation synergizes Z-NAs recognition and signal transduction
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Human
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A Subunit of the COP9 Signalosome, MoCsn6, Is Involved in Fungal Development, Pathogenicity, and Autophagy in Rice Blast Fungus
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Heme Oxygenase-1 and Its Metabolites Carbon Monoxide and Biliverdin, but Not Iron, Exert Antiviral Activity against Porcine Circovirus Type 3
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Pig
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A novel transcriptional repressor complex MYB22–TOPLESS–HDAC1 promotes rice resistance to brown planthopper by repressing F3′H expression
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Rice
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Junhao Li,et al
Overexpression of the receptor-like cytoplasmic kinase SZE1 positively regulates the PTI signaling pathway in Arabidopsis
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DDX5 inhibits type I IFN production by promoting degradation of TBK1 and disrupting formation of TBK1− TRAF3 complex
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IP
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Human
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Gao, X. K., Rao, X. S., Cong, X. X., Sheng, Z. K., Sun, Y. T., Xu, S. B., Wang, J. F., Liang, Y. H., Lu, L. R., Ouyang, H., Ge, H., Guo, J. S., Wu, H. J., Sun, Q. M., Wu, H. B., Bao, Z., Zheng, L. L., & Zhou, Y. T.
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