GFP Recombinant Rabbit Monoclonal Antibody [SY0243] (ET1607-31)
Catalog# ET1607-31
Rabbit Monoclonal to GFP
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WB
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IF-Cell
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IF-Tissue
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IP
Lysates/proteins at 50 ng/Lane.
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-31) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
GFP Recombinant Rabbit Monoclonal Antibody [SY0243] (ET1607-31)
Immunogen
Synthetic peptide within aequorea victoria gfp aa 1-50 / 238.
Host
Rabbit
Positive Control
K562 cell lysates transfected with GFP.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY0243
APPLICATION DILUTION
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WB
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1:1,000-1:10,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IP
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2-5 µg/ml.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
27 kD
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GFP
SYNONYMS
GFP antibody;Green fluorescent protein antibody;yfp antibody
FUNCTION
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
CITATIONS
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Wu, X., Lai, Y., Rao, S., Lv, L., Ji, M., Han, K., Weng, J., Lu, Y., Peng, J., Lin, L., Wu, G., Chen, J., Yan, F., & Zheng, H.
Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.
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WB
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Nicotiana tabacum
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Citation
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Huang, P., Li, Y., Wang, J., Wang, Q., Huang, Z., Liu, X., Lin, F., & Lu, J.
Casein Kinase 2 Mediates Degradation of Transcription Factor Pcf1 during Appressorium Formation in the Rice Blast Fungus
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IP
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M.oryzae
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Citation
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-
Zhou, D., Yan, H., Yang, S., Zhang, Y., Xu, X., Cen, X., Lei, K., & Xia, H.
SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway
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WB
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Human
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Citation
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Tang, H. P., Huang, C., Hu, C. B., Li, H., Shao, T., Ji, J. F., Bai, J., Fan, D. D., Lin, A. F., Xiang, L. X., & Shao, J. Z.
Inhibitory Role of an Aeromonas hydrophila TIR Domain Effector in Antibacterial Immunity by Targeting TLR Signaling Complexes in Zebrafish. Frontiers in microbiology, 12, 694081.
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Co-IP
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Zebrafish
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Citation
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-
Gao, X. K., Rao, X. S., Cong, X. X., Sheng, Z. K., Sun, Y. T., Xu, S. B., Wang, J. F., Liang, Y. H., Lu, L. R., Ouyang, H., Ge, H., Guo, J. S., Wu, H. J., Sun, Q. M., Wu, H. B., Bao, Z., Zheng, L. L., & Zhou, Y. T.
Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes
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WB
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Mouse
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Citation
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-
Wang, J., Huang, Z., Huang, P., Wang, Q., Li, Y., Liu, X. H., Lin, F. C., & Lu, J.
The Plant Homeodomain Protein Clp1 Regulates Fungal Development, Virulence, and Autophagy Homeostasis in Magnaporthe oryzae
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WB
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Co-IP
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Magnaporthe oryzae
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Citation
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-
Meng, S., Jagernath, J. S., Luo, C., Shi, H., & Kou, Y.
MoWhi2 Mediates Mitophagy to Regulate Conidiation and Pathogenesis in Magnaporthe oryzae
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WB
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Magnaporthe oryzae
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Citation
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