PRODUCT CODE: M1210-1

GFP Mouse Monoclonal Antibody [A11-C11] (M1210-1)

Applications

  • ICC IHC-P

REACTIVITY

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+
Immunofluorescent staining of K562 cells transfected with GFP gene using anti-GFP Mouse mAb (Cat. # M1210-1).
  • Immunofluorescent staining of K562 cells transfected with GFP gene using anti-GFP Mouse mAb (Cat. # M1210-1).
  • Immunohistochemical analysis of paraffin- embedded testis tissue of OCT4-IRES-EGFP mouse(B6;129S4-Pou5f1tm2Jae/J) using anti-GFP Mouse mAb (Cat. # M1210-1).
Immunofluorescent staining of K562 cells transfected with GFP gene using anti-GFP Mouse mAb (Cat. # M1210-1).

Applications

  • ICC IHC-P

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

GFP Mouse Monoclonal Antibody [A11-C11] (M1210-1)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

K562 cells transfected with GFP

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A11-C11

PROPERTIES

Form

Liquid

Storage Condition

Store at -20 C. Stable for 12 months from date of receipt.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

MOLECULAR WEIGHT

26kDa

Isotype

IgG2b

APPLICATION DILUTION

  • ICC:1:500-1:1,000

  • IHC-P

  • 1:100

TARGET

UNIPROT #

PROTEIN NAME

GFP

SYNONYMS

GFP antibody; Green fluorescent protein antibody; yfp antibody

SEQUENCE SIMILARITIES

Belongs to the GFP family.

TISSUE SPECIFICITY

Photocytes.

POST-TRANSLATIONAL MODIFICATION

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.

CITATIONS

  • Li, Xiaohui et al.

    Tomato SlMKK2 and SlMKK4 contribute to disease resistance against Botrytis cinerea. | Bmc Plant Biology [2014]

  • Li, Xiaohui et al.

    Tomato SlRbohB, a member of the NADPH oxidase family, is required for disease resistance against Botrytis cinerea and tolerance to drought stress. | Frontiers in Plant Science [2015]

  • Cao, Huifang et al.

    Novel T3SS effector EseK in Edwardsiella piscicida is chaperoned by EscH and EscS to express virulence. | Cellular Microbiology [2018]

  • Xu, Wenting et al.

    Edwardsiella piscicida virulence effector trxlp promotes the NLRC4 inflammasome activation during infection. | Microbial Pathogenesis [2018]