GFP Mouse Monoclonal Antibody [2-D8] (EM30501)
Catalog# EM30501
Mouse Monoclonal to GFP
-
WB
-
IF-Cell
-
ELISA
-
FC
-
IP
-
Species independent
Specifications
Product Type
Mouse monoclonal primary
Product Name
GFP Mouse Monoclonal Antibody [2-D8] (EM30501)
Immunogen
Recombinant full length protein of aequorea victoria gfp.
Host
Mouse
Positive Control
Transfected K562 with GFP.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
2-D8
RRID
APPLICATION DILUTION
-
WB
-
1:2,000
-
IF-Cell
-
1:200
-
IP
-
2-5 µg/ml.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 27 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
GFP
SYNONYMS
GFP antibody;Green fluorescent protein antibody;yfp antibody
SEQUENCE SIMILARITIES
Belongs to the GFP family.
TISSUE SPECIFICITY
Photocytes.
POST-TRANSLATIONAL MODIFICATION
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
FUNCTION
Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
CITATIONS
-
Tang Y
Successful construction and stable expression of an anti-CD45RA scFv–EGFP fusion protein in Chinese hamster ovary cells
-
WB
-
Chinese hamster
-
Citation
-
-
Fengming Song
Overexpression of MoSM1,encoding for an immunity-inducing protein from Magnaporthe oryzae,in rice confers broad-spectrum resistance against fungal and bacterial diseases
-
WB
-
IF
-
Oryza sativa
-
Citation
-
-
HFE inhibits type I IFNs signaling by targeting the SQSTM1-mediated MAVS autophagic degradation
-
IP
-
WB
-
Human
-
Citation
-
-
Tao Zhihuan,et al
HD-ZIP IV Gene ROC1 Regulates Leaf Rolling and Drought Response Through Formation of Heterodimers with ROC5 and ROC8 in Rice
-
Co-IP
-
Species independent
-
Citation
-
-
Liang Xue,et al
Suppressing FXR promotes antiviral effects of bile acids via enhancing the interferon transcription
-
IF
-
WB
-
Mouse
-
Citation
-
-
The E3 ubiquitin ligase OsRGLG5 targeted by the Magnaporthe oryzae effector AvrPi9 confers basal resistance against rice blast
-
WB
-
Co-IP
-
Rice
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.