GFP Mouse Monoclonal Antibody [2-D8] (EM30501)
Catalog# EM30501
Mouse Monoclonal to GFP
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WB
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ICC
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ELISA
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IP
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FC
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IP
Specifications
Product Type
Mouse monoclonal primary
Product Name
GFP Mouse Monoclonal Antibody [2-D8] (EM30501)
Immunogen
Recombinant full length protein of aequorea victoria gfp.
Host
Mouse
Positive Control
Transfected K562 with GFP.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
2-D8
APPLICATION DILUTION
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WB
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1:2,000
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ICC
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1:200
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IP
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2-5 µg/ml.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
26 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
GFP
SYNONYMS
GFP antibody;Green fluorescent protein antibody;yfp antibody
SEQUENCE SIMILARITIES
Belongs to the GFP family.
TISSUE SPECIFICITY
Photocytes.
POST-TRANSLATIONAL MODIFICATION
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
FUNCTION
Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
CITATIONS
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Fengming Song
Overexpression of MoSM1,encoding for an immunity-inducing protein from Magnaporthe oryzae,in rice confers broad-spectrum resistance against fungal and bacterial diseases
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WB
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IF
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Oryza sativa
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Citation
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Wang, Zhujun et al.
Successful construction and stable expression of an anti-CD45RA scFv-EGFP fusion protein in Chinese hamster ovary cells. | Protein Expression and Purification [2014]
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WB
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Chinese hamster
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Citation
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