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PRODUCT CODE: EM30501

GFP Mouse Monoclonal Antibody [2-D8] (EM30501)

Applications

  • WB

  • ICC

  • ELISA

  • IP

  • FC

  • IP

REACTIVITY

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Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution.
  • Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution.
  • ICC staining GFP in transfected K562 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with EM30501 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1604-25 at 1/1000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.<br /> <br /> Lane 1: GFP Tag fusion protein lysate (input).<br /> Lane 2: M1210-1 IP in GFP Tag fusion protein lysate.<br /> Lane 3: EM30501 IP in GFP Tag fusion protein lysate.<br /> Lane 4: Mouse IgG instead of EM30501 in GFP Tag fusion protein lysate.<br /> <br /> Blocking/Dilution buffer: 5% NFDM/TBST
Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution.

Applications

  • WB

  • ICC

  • ELISA

  • IP

  • FC

  • IP

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

GFP Mouse Monoclonal Antibody [2-D8] (EM30501)

Immunogen

Recombinant full length protein of aequorea victoria gfp.

Host

Mouse

Positive Control

Transfected K562 with GFP.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2-D8

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:2,000

  • ICC

  • 1:200

  • IP

  • 2-5 µg/ml.

TARGET

UNIPROT #

P42212

PROTEIN NAME

GFP

SYNONYMS

GFP antibody; Green fluorescent protein antibody; yfp antibody

SEQUENCE SIMILARITIES

Belongs to the GFP family.

TISSUE SPECIFICITY

Photocytes.

POST-TRANSLATIONAL MODIFICATION

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

FUNCTION

Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.

CITATIONS

  • Fengming Song

    Overexpression of MoSM1,encoding for an immunity-inducing protein from Magnaporthe oryzae,in rice confers broad-spectrum resistance against fungal and bacterial diseases

  • Wang, Zhujun et al.

    Successful construction and stable expression of an anti-CD45RA scFv-EGFP fusion protein in Chinese hamster ovary cells. | Protein Expression and Purification [2014]