Geminin Rabbit Polyclonal Antibody (R1311-8)

UNIPROT #

O88513

PROTEIN NAME

Geminin

SYNONYMS

DNA replication inhibitor antibody;Gem antibody;GEMI_HUMAN antibody;Geminin antibody;Geminin DNA replication inhibitor antibody;GMNN antibody;OTTHUMP00000039393 antibody;RP3 369A17.3 antibody

SEQUENCE SIMILARITIES

Belongs to the geminin family.

DEVELOPMENTAL STAGE

Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated during mitosis. Phosphorylation at Ser-181 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity.

SUBCELLULAR LOCATION

Cytoplasm, nucleus

FUNCTION

Geminin is a nuclear protein that is present in most eukaryotics and highly conserved across species. Numerous functions have been elucidated for Geminin including roles in metazoan cell cycle, cellular proliferation, cell lineage commitment, and neural differentiation. Geminin is absent during G1 phase and accumulates through S, G2 phase and M phases of the cell cycle. Geminin levels drop at the metaphase / anaphase transition of mitosis when it is degraded by the Anaphase Promoting Complex (APC/C). During S phase, geminin is a positive regulator of DNA replication. In many cancer cell lines, inhibition of geminin by RNAi results in re-replication of portions of the genome, which leads to aneuploidy. In these cell lines, geminin knockdown leads to markedly slowed growth and apoptosis within several days. Recently, geminin has been found to be overexpressed in several malignancies and cancer cell lines.