Specific bands were detected for GATA3 full length (FL) at approximately 52 kDa and the splice form (SF) at approximately 39 kDa (as indicated).
Applications
-
WB
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
GATA3 Mouse Monoclonal Antibody [A3G6] (EM1902-23)
Immunogen
Synthetic peptide corresponding to n terminal of human gata3.
Host
Mouse
Positive Control
MCF-7 cell lysates, jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A3G6
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
Predicted band size 48 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
GATA3
SYNONYMS
GATA 3 antibody; GATA binding factor 3 antibody; GATA binding protein 3 antibody; GATA-binding factor 3 antibody; Gata3 antibody; GATA3_HUMAN antibody; HDR antibody; HDRS antibody; MGC2346 antibody; MGC5199 antibody; MGC5445 antibody; Trans acting T cell specific transcription factor GATA 3 antibody; Trans-acting T-cell-specific transcription factor GATA-3 antibody
TISSUE SPECIFICITY
T-cells and endothelial cells.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
GATA3 is a transcription factor that in humans is encoded by the GATA3 gene. Studies in animal models and humans indicate that it controls the expression of a wide range of biologically and clinically important genes. The GATA3 transcription factor is critical for the embryonic development of various tissues as well as for inflammatory and humoral immune responses and the proper functioning of the endothelium of blood vessels. GATA3 haploinsufficiency (i.e. lose of one or the two inherited GATA3 genes) results in a congenital disorder termed the Barakat syndrome. Current clinical and laboratory research is focusing on determining the benefits of directly or indirectly blocking the action of GATA3 in inflammatory and allergic diseases such as asthma. It is also proposed to be a clinically important marker for various types of cancer, particularly those of the breast. However, the role, if any, of GATA3 in the development of these cancers is under study and remains unclear.