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Gasdermin D (N terminal) Rabbit Polyclonal Antibody (ER1901-37)

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Catalog# ER1901-37

Rabbit Polyclonal to Gasdermin D (N terminal)

Tested for

  • WB

  • IHC-P

  • FC

  • IF-Cell

Reacts with

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Rabbit polyclonal primary

Product Name

Gasdermin D (N terminal) Rabbit Polyclonal Antibody (ER1901-37)

Immunogen

Recombinant protein within human gasdermin d aa 100-300.

Host

Rabbit

Positive Control

SiHa cell lysate, PC-3 cell lysate, Jurkat cell lysate, THP-1 cell lysate, human kidney tissue lysate, SiHa, rat stomach tissue, human tonsil tissue, human prostate carcinoma tissue, human esophagus tissue, mouse colon tissue, mouse intestine tissue, SiHa, PC-3M, RAW264.7, L6.

Conjugation

Unconjugated

Clonality

Polyclonal

RRID

AB_3069329

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:1,000

  • FC

  • 1:1,000

  • IF-Cell

  • 1:100

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Immunogen affinity purified.

Molecular Weight

Predicted band size: 53 kDa

Isotype

IgG

TARGET

UNIPROT #

P57764

PROTEIN NAME

Gasdermin D (N terminal)

SYNONYMS

1810036L03Rik antibody;DF 5L antibody;DF5L antibody;DFNA 5L antibody;DFNA5L antibody;FKSG 10 antibody;FKSG10 antibody;FLJ12150 antibody;Gasdermin antibody;Gasdermin D antibody;Gasdermin domain containing 1 antibody;Gasdermin domain containing protein 1 antibody;Gasdermin domain-containing protein 1 antibody;Gasdermin-D antibody;GasderminD antibody;GSDMD antibody;GSDMD_HUMAN antibody;GSDMDC 1 antibody;GSDMDC1 antibody

SEQUENCE SIMILARITIES

Belongs to the gasdermin family.

TISSUE SPECIFICITY

Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells.

POST-TRANSLATIONAL MODIFICATION

Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms) or CASP4 relieves autoinhibition and is sufficient to initiate pyroptosis. Cleavage at Asp-87 by CASP3.

SUBCELLULAR LOCATION

Cytosol. Secreted. Plasma membrane.

FUNCTION

Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine. Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis . Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity.

CITATIONS

  • Fatma Elnaghy,et al

    Diltiazem mitigates acute liver injury by targeting NFκB-TXNIP/NLRP3 axis in Rats: New insights beyond calcium channel blockade

  • Jiajun Wu,et al

    Glutamine mitigates oxidative stress-induced matrix degradation, ferroptosis, and pyroptosis in nucleus pulposus cells via deubiquitinating and stabilizing Nrf2

  • Liao Y, Deng C, Wang X

    VSIG4 ameliorates intestinal inflammation through inhibiting macrophages NLRP3 inflammasome and pyroptosis

  • Wei Shi,et al

    Copper induced cytosolic escape of mitochondrial DNA and activation of cGAS-STING-NLRP3 pathway-dependent pyroptosis in C8-D1A cells

  • Wang Jiabin,et al

    Comprehensive multi-omics analysis of pyroptosis for optimizing neoadjuvant immunotherapy in patients with gastric cancer

  • Feng, Z., Huang, Q., Zhang, X., Xu, P., Li, S., Ma, D., & Meng, Q.

    PPAR- γ Activation Alleviates Osteoarthritis through Both the Nrf2/NLRP3 and PGC-1 α/ Δψ m Pathways by Inhibiting Pyroptosis

  • Lai Yingying,et al

    Puerarin enhances TFEB-mediated autophagy and attenuates ROS-induced pyroptosis after ischemic injury of random-pattern skin flaps

  • Lu Wang,et al

    Puerarin Reduces Diabetic Nephropathy-Induced Podocyte Pyroptosis by Modulating the SIRT1/NLRP3/Caspase-1 Pathway

  • Wu Dengying,et al

    PPAR γ activation in chondrocytes alleviates glucocorticoid-induced oxidative stress, mitochondrial impairment, and pyroptosis via autophagic flow enhancement

  • Zhu, Y., Huang, G., Yang, Y., Yong, C., Yu, X., Wang, G., Yi, L., Gao, K., Tian, F., Qian, S., Zhou, E., & Zou, Y.

    Chinese Herbal Medicine Suyin Detoxification Granule Inhibits Pyroptosis and Epithelial-Mesenchymal Transition by Downregulating MAVS/NLRP3 to Alleviate Renal Injury

  • Wen Guo,et al

    Salidroside Ameliorates Macrophages Lipid Accumulation and Atherosclerotic Plaque by Inhibiting Hif-1α-Induced Pyroptosis

  • Ye, Y., Feng, Z., Tian, S., Yang, Y., Jia, Y., Wang, G., Wang, J., Bai, W., Li, J., & He, X.

    HBO Alleviates Neural Stem Cell Pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 Axis and Improves Neurogenesis after Oxygen Glucose Deprivation

  • Wolf–Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation

  • Cao Yumei,et al

    Caspase-1 Inhibition Ameliorates Photoreceptor Damage Following Retinal Detachment by Inhibiting Microglial Pyroptosis

  • Haojiang Duan,et al

    GBP1 promotes acute rejection after liver transplantation by inducing Kupffer cells pyroptosis

  • Pyroptosis predicts immunotherapy outcomes across multiple cancer types

  • Ma Xiao,et al

    Paeoniflorin inhibited GSDMD to alleviate ANIT-induced cholestasis via pyroptosis signaling pathway

  • Hwang, S., Kim, S. H., Yoo, K. H., Chung, M. H., Lee, J. W., & Son, K. H.

    Exogenous 8-hydroxydeoxyguanosine attenuates doxorubicin-induced cardiotoxicity by decreasing pyroptosis in H9c2 cardiomyocytes

  • Chuanting Xu

    Histone deacetylase-mediated silencing of PSTPIP2 expression contributes to aristolochic acid nephropathy-induced PANoptosis

  • An Minglin,et al

    PI3K/AKT signaling pathway associates with pyroptosis and inflammation in patients with endometriosis

  • Sun Dalin,et al

    Astragaloside IV Alleviates Podocyte Injury in Diabetic Nephropathy through Regulating IRE-1α/NF-κ B/NLRP3 Pathway

  • Achyranthoside D attenuates chondrocyte loss and inflammation in osteoarthritis via targeted regulation of Wnt3a

  • Tojo, K., Yamamoto, N., Tamada, N., Mihara, T., Abe, M., Nishii, M., Takeuchi, I., & Goto, T.

    Early alveolar epithelial cell necrosis is a potential driver of COVID-19-induced acute respiratory distress syndrome

  • Wang Quanzhen,et al

    LL-37 improves sepsis-induced acute lung injury by suppressing pyroptosis in alveolar epithelial cells

  • Yao, M., Wang, Z., Jiang, L., Wang, L., Yang, Y., Wang, Q., Qian, X., Zeng, W., Yang, W., Liang, R., & Qian, J.

    Oxytocin ameliorates high glucose- and ischemia/reperfusion-induced myocardial injury by suppressing pyroptosis via AMPK signaling pathway

  • Jin Hui,et al

    Tranilast alleviates skin inflammation and fibrosis in rosacea-like mice induced by long-term exposure to LL-37

  • Nie, L., Zhao, P., Yue, Z., Zhang, P., Ji, N., Chen, Q., & Wang, Q.

    Diabetes induces macrophage dysfunction through cytoplasmic dsDNA/AIM2 associated pyroptosis. Journal of leukocyte biology, 10.1002/JLB.3MA0321-745R. Advance online publication.

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