Lane 1: SiHa cell lysate
Lane 2: human kidney tissue lysate
Rabbit polyclonal primary
Gasdermin D (N terminal) Rabbit Polyclonal Antibody (ER1901-37)
Recombinant protein within human gasdermin d aa 100-300.
SiHa cell lysates, human kidney tissue lysates, SiHa, rat stomach tissue, human tonsil tissue, human prostate carcinoma tissue, human esophagus tissue, Mouse colon tissue.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein affinity purified.
Gasdermin D (N terminal)
1810036L03Rik antibody; DF 5L antibody; DF5L antibody; DFNA 5L antibody; DFNA5L antibody; FKSG 10 antibody; FKSG10 antibody; FLJ12150 antibody; Gasdermin antibody; Gasdermin D antibody; Gasdermin domain containing 1 antibody; Gasdermin domain containing protein 1 antibody; Gasdermin domain-containing protein 1 antibody; Gasdermin-D antibody; GasderminD antibody; GSDMD antibody; GSDMD_HUMAN antibody; GSDMDC 1 antibody; GSDMDC1 antibody
Belongs to the gasdermin family.
Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells.
Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms) or CASP4 relieves autoinhibition and is sufficient to initiate pyroptosis. Cleavage at Asp-87 by CASP3.
Cytosol. Secreted. Plasma membrane.
Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine. Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis . Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity.