GAP43 Recombinant Rabbit Monoclonal Antibody [SC60-06] (ET1610-94)
Catalog# ET1610-94
Rabbit Monoclonal to GAP43
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Western blot analysis of GAP43 on different lysates with Rabbit anti-GAP43 antibody (ET1610-94) at 1/100,000 dilution and competitor's antibody at 1/100,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: A549 cell lysate (negative)
Lane 3: Neuro-2a cell lysate
Lane 4: Mouse lung tissue lysate (negative)
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 43/45 kDa
Exposure time: 3 minutes 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-94) at 1/100,000 dilution and competitor's antibody at 1/100,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
GAP43 Recombinant Rabbit Monoclonal Antibody [SC60-06] (ET1610-94)
Immunogen
Synthetic peptide within human gap43 aa 189-238 / 238.
Host
Rabbit
Positive Control
SH-SY5Y cell lysate, Neuro-2a cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human glioma tissue, Neuro-2a, rat hippocampus tissue lysate, mouse hippocampus tissue lysate, mouse brain tissue, rat brain tissue, rat hippocampus tissue, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC60-06
RRID
APPLICATION DILUTION
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WB
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1:20,000-1:100,000
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IF-Cell
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1:500
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IF-Tissue
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1:500-1:1,000
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IHC-P
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1:10,000-1:15,000
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 25 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GAP43
SYNONYMS
Axonal membrane protein GAP 43 antibody;Axonal membrane protein GAP-43 antibody;B 50 antibody;Calmodulin binding protein P 57 antibody;F1 antibody;GAP 43 antibody;GAP43 antibody;Growth Associated Protein 43 antibody;Growth-associated protein 43 antibody;Nerve Growth Related Peptide antibody;Nerve growth related peptide GAP43 antibody;NEUM_HUMAN antibody;Neural phosphoprotein B 50 antibody;Neural phosphoprotein B-50 antibody;Neuromodulin antibody;Neuron growth associated protein 43 antibody;PP46 antibody;Protein F1 antibody;QtrA-11580 antibody;QtrA-13071 antibody
SEQUENCE SIMILARITIES
Belongs to the neuromodulin family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated (By similarity). Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity (By similarity).; Palmitoylated by ZDHHC3 (By similarity). Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.
SUBCELLULAR LOCATION
Cell membrane, growth cone membrane, filopodium membrane, Cytoplasm, synapse, perikaryon, dendrite, axon.
FUNCTION
GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity. The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. GAP43, the consensus choice for its designation, is a nervous system-specific protein that is attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4, though it can exist in the non-bound form in the cytoplasm. This dual sequence enables the association of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] or PIP2, with actin, facilitating the latter's polymerization thereby regulating neuronal structure. GAP-43 is also a protein kinase C (PKC) substrate. Phosphorylation of serine-41 on GAP-43 by PKC regulates neurite formation, regeneration, and synaptic plasticity.
CITATIONS
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Yun-jin Zang;Bei Zhang
NLRP3 inflammasome is involved in nerve recovery after sciatic nerve injury
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WB
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IHC
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Mouse
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Citation
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