PRODUCT CODE: ET1702-48

Galectin 3 Recombinant Rabbit Monoclonal Antibody [JF39-10] (ET1702-48)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of Galectin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: NIH/3T3 cell lysate<br />
Lane 2: Hela cell lysate<br />
Lane 3: THP-1 cell lysate
  • Western blot analysis of Galectin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: NIH/3T3 cell lysate<br />
Lane 2: Hela cell lysate<br />
Lane 3: THP-1 cell lysate
  • ICC staining of Galectin 3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Galectin 3 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Flow cytometric analysis of Galectin 3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-48, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Galectin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: THP-1 cell lysate

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Galectin 3 Recombinant Rabbit Monoclonal Antibody [JF39-10] (ET1702-48)

Immunogen

Synthetic peptide within n-terminal human galectin 3.

Host

Rabbit

Positive Control

NIH/3T3 cell lysate, Hela cell lysate, THP-1 cell lysate, Hela, NIH/3T3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF39-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Galectin 3

SYNONYMS

35 kDa lectin antibody; Carbohydrate binding protein 35 antibody; Carbohydrate-binding protein 35 antibody; CBP 35 antibody; CBP35 antibody; Gal-3 antibody; GAL3 antibody; Galactose-specific lectin 3 antibody; Galactoside-binding protein antibody; GALBP antibody; Galectin 3 internal gene,included antibody; Galectin-3 antibody; Galectin3 antibody; GALIG antibody; GBP antibody; IgE binding protein antibody; IgE-binding protein antibody; L 31 antibody; L 34 antibody; L-31 antibody; L-34 galactoside-binding lectin antibody; L31 antibody; Laminin-binding protein antibody; Lectin L-29 antibody; Lectin, galactose binding, soluble 3 antibody; LEG3_HUMAN antibody; LGALS2 antibody; LGALS3 antibody; MAC 2 antigen antibody; Mac-2 antibody; Mac-2 antigen antibody; MAC2 antibody; Macrophage galactose-specific lectin antibody; MGC105387 antibody

TISSUE SPECIFICITY

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.

SUBCELLULAR LOCATION

Nucleus, Secreted, Cytoplasm.

FUNCTION

Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) inter- actions and play a role in tumor progression, pre-mRNA splicing and apop-tosis. The galectin-3 protein, also known as Mac-2, hMac-2, GALBP, CBP35 or LGALS3, contains a single carbohydrate binding domain, which binds galactose-containing glycoconjugates. Galectin-3 is expressed in colonic and intestinal epithelium, inflammatory macrophages, papillary and follicular carcinomas, neoplastic astrocytes and some B and T lymphocytes. Upregulated expression of galectin-3 is involved in cancer progression and metastasis. Galectin-3 mediates the endocytosis of β1 Integrins in a lactose-dependent manner and is associated with thyroid malignancy and Crohn's disease. It may also be used as a marker for diagnosing cases involving Hurthle cell adenomas and carcinomas.