PRODUCT CODE: ET1705-83

Galectin 1 Recombinant Rabbit Monoclonal Antibody [JM13-37] (ET1705-83)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Rat

  • Mouse

Western blot analysis of Galectin 1 on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Galectin 1 on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of Galectin 1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-Galectin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-83, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Galectin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-83, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Galectin 1 was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-83, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Galectin 1 on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Rat

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Galectin 1 Recombinant Rabbit Monoclonal Antibody [JM13-37] (ET1705-83)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human skeletal muscle tissue lysates, A431, rat stomach tissue, human placenta tissue, NIH/3T3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM13-37

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:400-1:800

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Galectin 1

SYNONYMS

14 kDa laminin-binding protein antibody; 14 kDa lectin antibody; Beta galactoside binding lectin antibody; Beta galactoside binding lectin L 14 I antibody; beta galactoside binding protein antibody; Beta-galactoside-binding lectin L-14-I antibody; DKFZp686E23103 antibody; Gal 1 antibody; Gal-1 antibody; GAL1 antibody; Galaptin antibody; Galbp antibody; Galectin antibody; Galectin-1 antibody; Galectin1 antibody; GBP antibody; HBL antibody; HLBP14 antibody; HPL antibody; L 14.5 antibody; L-14.5 antibody; L14 antibody; Lactose binding lectin 1 antibody; Lactose-binding lectin 1 antibody; Lect14 antibody; Lectin galactoside binding soluble 1 antibody; Lectin galactoside-binding soluble 1 antibody; LEG1_HUMAN antibody; LGALS 1 antibody; LGALS1 antibody; Lgals1 lectin galactose binding soluble 1 antibody; MAPK activating protein MP12 antibody; Putative MAPK activating protein MP12 antibody; Putative MAPK-activating protein PM12 antibody; S Lac lectin 1 antibody; S-Lac lectin 1 antibody

TISSUE SPECIFICITY

Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

SUBCELLULAR LOCATION

Extracellular matrix.

FUNCTION

Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. Specifically, Galectin-1 is an autocrine regulator of cell-proliferation that plays a role in the maintenance of G0 and in the control of G2 traverse. Galectin-1, also known as LGALS1, is the protein product of a single gene linked to human chromosome 22q13.1. The Galectin-1 protein contains 135 amino acids, a single internal EcoRI site and a polyadenylation signal. Galectin-1 can localize to both intracellular and extracellular space. Galectin-1 is expressed in human placenta, human lung, HL-6, HepG2 and CEM cells.