GADD34 Mouse Monoclonal Antibody [A4D7] (EM1902-26)

UNIPROT #

O75807

PROTEIN NAME

GADD34

SYNONYMS

Apoptosis associated protein antibody;GADD 34 antibody;Growth arrest and DNA damage inducible 34 antibody;Growth arrest and DNA damage-inducible protein GADD34 antibody;Myeloid differentiation primary response protein MyD116 homolog antibody;Ppp1r15a antibody;PR15A_HUMAN antibody;Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody;Protein phosphatase 1 regulatory subunit 15A antibody

SEQUENCE SIMILARITIES

Belongs to the PPP1R15 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at multiple Ser/Thr residues. Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity. Phosphorylation at Tyr-262 enhances proteasomal degradation, this position is dephosphorylated by PTPN2.; Polyubiquitinated. Exhibits a rapid proteasomal degradation with a half-life under 1 hour, ubiquitination depends on endoplasmic reticulum association.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane, Mitochondrion outer membrane.

FUNCTION

Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene. The Gadd34/MyD116 gene was originally discovered as a member in a set of gadd and MyD mammalian genes encoding acidic proteins that synergistically suppress cell growth. Later on it has been characterized as a gene playing a role in ER stress-induced cell death, being a target of ATF4 that plays a role in ER-mediated cell death via promoting protein dephosphorylation of eIF2α and reversing translational inhibition. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.