Positive control:
Lane 1: 293 cell lysate
Lane 2: SHSY5Y cell lysate
Applications
-
WB
-
IHC-P
-
ICC
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
GABARAPL2 Rabbit Polyclonal Antibody (ER1901-73)
Immunogen
Recombinant protein within human gabarapl2 aa 1-117 / 117.
Host
Rabbit
Positive Control
293 cell lysate, SHSY5Y cell lysate, SHSY5Y, Siha, Rat cerebellum tissue, Human prostate tissue, Mouse small intestine tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
14 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2000
-
IHC-P
-
1:50-1:200
-
ICC
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
GABARAPL2
SYNONYMS
ATG8 antibody; ATG8C antibody; FLC3A antibody; GABA(A) receptor-associated protein-like 2 antibody; Gabarapl2 antibody; Gamma-aminobutyric acid receptor-associated protein-like 2 antibody; Ganglioside expression factor 2 antibody; GATE-16 antibody; GATE16 antibody; GBRL2_HUMAN antibody; GEF-2 antibody; GEF2 antibody; General protein transport factor p16 antibody; Golgi-associated ATPase enhancer of 16 kDa antibody; MAP1 light chain 3-related protein antibody
SEQUENCE SIMILARITIES
Belongs to the ATG8 family.
TISSUE SPECIFICITY
Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.
POST-TRANSLATIONAL MODIFICATION
The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL2-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL2-II. ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.; The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
SUBCELLULAR LOCATION
Cytoplasmic vesicle. Golgi apparatus.
FUNCTION
Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.