FTO Recombinant Rabbit Monoclonal Antibody [JM98-30] (ET1705-89)
Catalog# ET1705-89
Rabbit Monoclonal to FTO
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Human lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 55 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-89) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
FTO Recombinant Rabbit Monoclonal Antibody [JM98-30] (ET1705-89)
Immunogen
Synthetic peptide within human fto aa 1-50 / 505.
Host
Rabbit
Positive Control
HEK-293 cell lysate, HeLa cell lysate, Human lung tissue lysate, human lung tissue, human placenta tissue, human uterus tissue, HEK-293, NIH/3T3, L6.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM98-30
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:200-1:1,000
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IF-Cell
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 58 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
FTO
SYNONYMS
AlkB homolog 9 antibody;ALKBH9 antibody;Alpha-ketoglutarate-dependent dioxygenase FTO antibody;AW743446 antibody;Fat mass and obesity-associated protein antibody;FATSO, MOUSE, HOMOLOG OF antibody;Fto antibody;FTO_HUMAN antibody;GDFD antibody;KIAA1752 antibody;mKIAA1752 antibody;Protein fatso antibody
SEQUENCE SIMILARITIES
Belongs to the fto family.
TISSUE SPECIFICITY
Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary. Highly expressed in highly expressed in acute myeloid leukemias (AML) with t(11;11)(q23;23) with KMT2A/MLL1 rearrangements, t(15;17)(q21;q21)/PML-RARA, FLT3-ITD, and/or NPM1 mutations.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
FTO, also known as Fatso or KIAA1752, is a 505 amino acid protein that has an N-terminal nuclear localization signal. Expressed in a variety of tissues, with highest levels present in brain and pancreatic tissue, Fatso exists as four alternatively spliced isoforms, one of which is associated with a predisposition to childhood and adult obesity. Due to its involvement in the development of obesity, Fatso is associated with an increased BMI and may be involved in the pathogenesis of type 2 diabetes. The gene encoding Fatso maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition.
CITATIONS
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Cao, X., Shu, Y., Chen, Y., Xu, Q., Guo, G., Wu, Z., Shao, M., Zhou, Y., Chen, M., Gong, Y., Li, C., Shi, Y., & Bu, H.
Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cellular and molecular gastroenterology and hepatology, S2352-345X(21)00072-2. Advance online publication.
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WB
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Mouse
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Citation
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