Western blot analysis of Frizzled-5 on human ES cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Rabbit polyclonal primary
Frizzled-5 Rabbit Polyclonal Antibody (0905-3)
Synthetic peptide within human frizzled-5 aa 143-192 / 585.
Belongs to the G-protein coupled receptor Fz/Smo family.
Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome.
Cell membrane.Golgi apparatus. Membrane. Synapse.
Frizzled-5 belongs to the G-protein coupled receptor Fz/Smo family, contains 585 amino acids. It is localized at the plasma membrane and also found at the Golgi. Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.