PRODUCT CODE: R1511-36

FPR1 Rabbit Polyclonal Antibody (R1511-36)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of FPR1 on different cell lysate using anti-FPR1 antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: NCCIT Lane 2: MEF<br />
Lane 3: HES
  • Western blot analysis of FPR1 on different cell lysate using anti-FPR1 antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: NCCIT Lane 2: MEF<br />
Lane 3: HES
  • ICC staining FPR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining FPR1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining FPR1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FPR1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FPR1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with FPR1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of FPR1 on different cell lysate using anti-FPR1 antibody at 1/500 dilution.
Positive control:
Lane 1: NCCIT Lane 2: MEF
Lane 3: HES

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

FPR1 Rabbit Polyclonal Antibody (R1511-36)

Immunogen

Peptide

Host

Rabbit

Positive Control

NCCIT, MEF, HES, Hela, HepG2, MCF-7, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

FPR1

SYNONYMS

fMet Leu Phe receptor antibody; fMet-Leu-Phe receptor antibody; FMLP antibody; fMLP receptor antibody; FMLPR antibody; Formyl peptide receptor 1 antibody; FPR 1 antibody; FPR antibody; FPR receptor antibody; FPR1 antibody; FPR1_HUMAN antibody; N formyl peptide chemoattractant receptor antibody; N formyl peptide receptor antibody; N formylpeptide chemoattractant receptor antibody; N-formyl peptide receptor antibody; N-formylpeptide chemoattractant receptor antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Neutrophils.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated; which is necessary for desensitization.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

High affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.