PRODUCT CODE: ET1702-44

FOXP1 Recombinant Rabbit Monoclonal Antibody [JF1017] (ET1702-44)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of FOXP1 on different lysates using anti-FOXP1 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela            <br />
Lane 2: Jurkat <br />
Lane 3: MCF-7
  • Western blot analysis of FOXP1 on different lysates using anti-FOXP1 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela            <br />
Lane 2: Jurkat <br />
Lane 3: MCF-7
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-FOXP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXP1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with FOXP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of FOXP1 on different lysates using anti-FOXP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: MCF-7

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

FOXP1 Recombinant Rabbit Monoclonal Antibody [JF1017] (ET1702-44)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, MCF-7, Hela, human colon cancer tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF1017

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

77/75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

FOXP1

SYNONYMS

12CC4 antibody; FLJ23741 antibody; Fork head related protein like B antibody; Forkhead box P1 antibody; Forkhead box protein P1 antibody; FOX P1 antibody; FOXP 1 antibody; foxp1 antibody; FOXP1_HUMAN antibody; Glutamine rich factor 1 antibody; hFKH1B antibody; HSPC215 antibody; MGC12942 antibody; MGC88572 antibody; MGC99551 antibody; QRF 1 antibody; QRF1 antibody

TISSUE SPECIFICITY

Isoform 8 is specifically expressed in embryonic stem cells.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. The gene encoding human FOXP1 maps to chromosome 3p14.1. The gene encoding human FOXP2 maps to chromosome 7q31. The gene encoding FOXP3, a third member of this family, maps to chromosome Xp11.23-Xq13.3. Mutations in this gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein, also known as scurfin, is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T-cell activation.