FOXO4 Recombinant Rabbit Monoclonal Antibody [SD0817] (ET1612-66)

UNIPROT #

P98177

PROTEIN NAME

FOXO4

SYNONYMS

AFX antibody;AFX1 antibody;Afxh antibody;ALL1-fused gene from X chromosome antibody;Fork head domain transcription factor AFX1 antibody;Forkhead box O4 antibody;Forkhead box protein O4 antibody;FOXO 4 antibody;Foxo4 antibody;FOXO4_HUMAN antibody;MGC117660 antibody;MGC120490 antibody;Mixed lineage leukemia, translocated to, 7 antibody;MLLT7 antibody;Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody;Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody;RGD1561201 antibody

TISSUE SPECIFICITY

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

POST-TRANSLATIONAL MODIFICATION

Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.; Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK.; Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

FKHR (for forkhead in rhabdomyosarcoma), FKHRL1, and AFX1 are members of a subfamily of the forkhead family of transcription factors. AFX1, also known as FoxO4, is expressed in a wide variety of tissues and, like other FKHR proteins, AFX1 contains a single forkhead domain and serine-proline-rich region, which mediate DNA binding. AFX1-mediated transcriptional activation is regulated by the serine/threonine kinase Akt1, which phosphorylates AFX1 and in turn, sequesters AFX1 in the cytosol, thereby blocking nuclear localization and DNA binding. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. Additionally, the t(x;11) translocation of the AFX1 gene, which involves the fusion of a serine-proline-rich sequence of AFX1 to the carboxy terminus of a truncated MLL, results in acute lymphocytic leukemia.