FOXA2 Recombinant Rabbit Monoclonal Antibody [JM10-64] (ET1703-76)
Catalog# ET1703-76
Rabbit Monoclonal to FOXA2
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WB
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IF-Cell
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IHC-P
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IHC-Fr
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Species: Mouse
Site: Liver
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
FOXA2 Recombinant Rabbit Monoclonal Antibody [JM10-64] (ET1703-76)
Immunogen
Synthetic peptide within human foxa2 aa 1-50/457.
Host
Rabbit
Positive Control
HT-29 cell lysate, A549 cell lysate, HepG2 cell lysate, mouse liver tissue, rat liver tissue, HT-29.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM10-64
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
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IHC-Fr
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
FOXA2
SYNONYMS
Forkhead box A2 antibody;Forkhead box protein A2 antibody;FOX A2 antibody;foxa2 antibody;FOXA2_HUMAN antibody;Hepatic nuclear factor 3 beta antibody;Hepatocyte nuclear factor 3 antibody;Hepatocyte nuclear factor 3 beta antibody;Hepatocyte nuclear factor 3-beta antibody;HNF 3B antibody;HNF-3-beta antibody;HNF-3B antibody;HNF3B antibody;MGC19807 antibody;TCF 3B antibody;TCF-3B antibody;TCF3B antibody;Transcription factor 3B antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
SUBCELLULAR LOCATION
Nucleus. Cytoplasm.
FUNCTION
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
CITATIONS
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Lv, J., Yi, Y., Qi, Y., Yan, C., Jin, W., Meng, L., Zhang, D., & Jiang, W.
Mitochondrial homeostasis regulates definitive endoderm differentiation of human pluripotent stem cells
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IF
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Human
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Citation
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Yi Ying,et al
Fatty acid synthesis and oxidation regulate human endoderm differentiation by mediating SMAD3 nuclear localization via acetylation
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IF-Cell
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Human
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Citation
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Meng, Y., Zhang, T., Zheng, R., Ding, S., Yang, J., Liu, R., Jiang, Y., & Jiang, W.
Depletion of Demethylase KDM6 Enhances Early Neuroectoderm Commitment of Human PSCs
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IF
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Mouse
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Citation
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Ding, S., Lan, X., Meng, Y., Yan, C., Li, M., Li, X., Chen, J., & Jiang, W.
CHD8 safeguards early neuroectoderm differentiation in human ESCs and protects from apoptosis during neurogenesis
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IF
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Human
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Citation
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