PRODUCT CODE: ER1803-66

FNDC3A Rabbit Polyclonal Antibody (ER1803-66)

  • Zebrafish

Applications

  • IF

  • IHC-P

REACTIVITY

  • Zebrafish

Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (ER1803-66) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
  • Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (ER1803-66) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-FNDC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-66) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (ER1803-66) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

Applications

  • IF

  • IHC-P

REACTIVITY

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

FNDC3A Rabbit Polyclonal Antibody (ER1803-66)

Immunogen

Recombinant protein within zebrafish fndc3a aa 97-329 / 619.

Host

Rabbit

Positive Control

Zebrafish.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

67 kDa

Isotype

IgG

APPLICATION DILUTION

  • IF:1:50-1:200

  • IHC-P:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

FNDC3A

SYNONYMS

Fibronectin type III domain containing protein 3a antibody; FNDC3 antibody; HUGO antibody; KIAA0970 antibody

SUBCELLULAR LOCATION

Golgi apparatus, Membrane.

FUNCTION

Fibronectin type III domain containing 7, or, FNDC7, is a human gene. Other names include: Fibronectin type III domain-containing protein 7. The encoded protein has an amino acid length of 733 and a mass of 78.2 kDa. Other isoforms may exist. This gene shares homology with other species, including: Mouse, Rat, Zebrafish, Frog, Chicken.