PRODUCT CODE: ET7108-93

FKBP52 Recombinant Rabbit Monoclonal Antibody [JG96-30] (ET7108-93)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of FKBP52 on 293 (1) and SK-Br-3 (2) cell lysate using anti-FKBP52 antibody at 1/2,000 dilution.
  • Western blot analysis of FKBP52 on 293 (1) and SK-Br-3 (2) cell lysate using anti-FKBP52 antibody at 1/2,000 dilution.
  • ICC staining FKBP52 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining FKBP52 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-FKBP52 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-FKBP52 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FKBP52 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with FKBP52 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of FKBP52 on 293 (1) and SK-Br-3 (2) cell lysate using anti-FKBP52 antibody at 1/2,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

FKBP52 Recombinant Rabbit Monoclonal Antibody [JG96-30] (ET7108-93)

Immunogen

Recombinant protein within human fkbp52 aa 1-200.

Host

Rabbit

Positive Control

293, SK-Br-3, 293T, MCF-7, human prostate cancer tissue, human esophagus tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG96-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

FKBP52

SYNONYMS

51 kDa FK506-binding protein antibody; 52 kDa FK506 binding protein antibody; 52 kDa FK506-binding protein antibody; 52 kDa FKBP antibody; 59 kDa immunophilin antibody; FK506 binding protein 4 antibody; FK506-binding protein 4 antibody; FKBP 4 antibody; FKBP 52 antibody; FKBP 59 antibody; FKBP-4 antibody; FKBP-52 antibody; FKBP4 antibody; FKBP4_HUMAN antibody; FKBP51 antibody; FKBP52 protein antibody; FKBP59 antibody; HBI antibody; Hsp 56 antibody; HSP binding immunophilin antibody; HSP-binding immunophilin antibody; Hsp56 antibody; Immunophilin FKBP52 antibody; N-terminally processed antibody; p52 antibody; p59 antibody; p59 protein antibody; Peptidyl prolyl cis trans isomerase antibody; Peptidyl-prolyl cis-trans isomerase FKBP4 antibody; Peptidylprolyl cis trans isomerase antibody; PPIase antibody; PPIase FKBP4 antibody; Rotamase antibody; T cell FK506 binding protein 59kD antibody

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by CK2 results in loss of HSP90 binding activity.

SUBCELLULAR LOCATION

Cytoplasm. Cytoskeleton. Microtubule. Mitochondrion. Nucleus.

FUNCTION

Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.