PRODUCT CODE: ET7107-64

FKBP51 Recombinant Rabbit Monoclonal Antibody [JB95-39] (ET7107-64)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of FKBP51 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Daudi cell lysate<br />
Lane 2: Hela cell lysate
  • Western blot analysis of FKBP51 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Daudi cell lysate<br />
Lane 2: Hela cell lysate
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FKBP51 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FKBP51 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-FKBP51 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of FKBP51 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of FKBP51 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Daudi cell lysate
Lane 2: Hela cell lysate

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

FKBP51 Recombinant Rabbit Monoclonal Antibody [JB95-39] (ET7107-64)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Daudi cell lysate, Hela cell lysate, human tonsil tissue, human kidney tissue, rat lung tissue, Daudi.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB95-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

51 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

FKBP51

SYNONYMS

51 kDa FK506 binding protein 5 antibody; 51 kDa FK506 binding protein antibody; 51 kDa FK506-binding protein antibody; 51 kDa FKBP antibody; 54 kDa progesterone receptor associated immunophilin antibody; 54 kDa progesterone receptor-associated immunophilin antibody; AIG 6 antibody; AIG6 antibody; Androgen regulated protein 6 antibody; Androgen-regulated protein 6 antibody; FF1 antigen antibody; FK506 binding protein 5 antibody; FK506-binding protein 5 antibody; FKBP 5 antibody; FKBP 51 antibody; FKBP 54 antibody; FKBP-5 antibody; FKBP-51 antibody; FKBP5 antibody; FKBP5_HUMAN antibody; FKBP54 antibody; HSP90 binding immunophilin antibody; HSP90-binding immunophilin antibody; MGC111006 antibody; OTTHUMP00000016268 antibody; P54 antibody; Peptidyl prolyl cis trans isomerase antibody; Peptidyl-prolyl cis-trans isomerase FKBP5 antibody; Peptidylprolyl cis trans isomerase antibody; PPIase antibody; PPIase FKBP5 antibody; Ptg 10 antibody; Ptg10 antibody; Rotamase antibody; T cell FK506 binding protein antibody

TISSUE SPECIFICITY

Widely expressed, enriched in testis compared to other tissues.

POST-TRANSLATIONAL MODIFICATION

Acetylation impairs ability to promote interaction between Akt/AKT1 and PHLPP1. Deacetylation by SIRT7 promotes interaction between Akt/AKT1 and PHLPP1, leading to suppress Akt/AKT1 activation.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

The immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases that bind to and mediate the effects of immunosuppressive drugs, such as cyclosporin, FK506 and rapamycin. Several related immunophilins, FKBP12, FKBP51 and FKBP52, are characterized as cytosolic FK506-binding proteins, and following ligand binding, they functionally inhibit the phosphatase activity of calcineurin. The ubiquitously expressed FKBP12 also associates with the cytoplasmic domain of the TGFβ-type I receptor, where it stabilizes the inactive conformation of the receptor and blocks the activation of the TGFβ pathway. FKBP51 and FKBP52 are two highly related proteins. FKBP51 is predominantly expressed in T cells and is induced by glucocorticoids. FKBP51 mediates the effects of FK506 and rapamycin by inhibiting intracellular calcineurin activity, and by blocking T-cell activation and proliferation. FKBP52, known also as FKBP-59 or heat shock protein 56, is expressed in a variety of tissues and can also associate with the heat shock protein (hsp90) in mature steroid receptor complexes (6,8).