PRODUCT CODE: ET1703-55

FKBP12 Recombinant Rabbit Monoclonal Antibody [JM11-09] (ET1703-55)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of FKBP12 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SH-SY5Y cell lysate<br />
Lane 2: U937 cell lysate
  • Western blot analysis of FKBP12 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SH-SY5Y cell lysate<br />
Lane 2: U937 cell lysate
  • Western blot analysis of FKBP12 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • ICC staining of FKBP12 in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of FKBP12 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of FKBP12 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of FKBP12 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SH-SY5Y cell lysate
Lane 2: U937 cell lysate

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

FKBP12 Recombinant Rabbit Monoclonal Antibody [JM11-09] (ET1703-55)

Immunogen

Synthetic peptide within human fkbp12 aa 76-108 / 108.

Host

Rabbit

Positive Control

SH-SY5Y, V937, HepG2, A431, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

12 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

FKBP12

SYNONYMS

12 kDa FK506-binding protein antibody; 12 kDa FKBP antibody; Calstabin 1 antibody; FK506 binding protein 1 antibody; FK506 binding protein 12 antibody; FK506 binding protein 1A (12kD) antibody; FK506 binding protein 1A 12kDa antibody; FK506 binding protein 1A antibody; FK506 binding protein T cell 12 kD antibody; FK506 binding protein, T cell, 12 kD antibody; FK506 binding protein12 antibody; FK506-binding protein 1A antibody; FKB1A_HUMAN antibody; FKBP 12 antibody; FKBP 1A antibody; FKBP-12 antibody; FKBP-1A antibody; FKBP1 antibody; FKBP12 antibody; FKBP12 Exip3 antibody; FKBP12C antibody; fkbp1a antibody; Immunophilin FKBP12 antibody; Peptidyl prolyl cis trans isomerase antibody; Peptidyl-prolyl cis-trans isomerase FKBP1A antibody; PKC12 antibody; PKCI2 antibody; PPIase antibody; PPIase FKBP1A antibody; Protein kinase C inhibitor 2 antibody; Rotamase antibody

SEQUENCE SIMILARITIES

Belongs to the FKBP-type PPIase family. FKBP1 subfamily.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases which bind to and mediate the effects of immunosuppressive drugs such as Cyclosporin, FK506 and Rapamycin. FKBP12.6, also known as FK506-binding protein 1B, is a 108 amino acid immunophilin belonging to the FKBP-type PPIase family. Subcellularly localized to the cytoplasm, FKBP12.6 binds to RyR in cardiac muscle sarcoplasmic reticulum and possibly plays a unique physiological role in excitation-contraction coupling in cardiac muscle. FKBP12.6 also catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Ubiquitously expressed, FKBP12.6 is found at highest levels in brain and thymus. FKBP12.6 is expressed as two isoforms produced by alternative splicing.