PRODUCT CODE: ET1703-55

FKBP12 Recombinant Rabbit Monoclonal Antibody [JM11-09] (ET1703-55)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis of FKBP12 on different cells lysates using anti-FKBP12 antibody at 1/500 dilution.<br />
Positive control: <br />
Line 1: SH-SY5Y           <br />
Line 2:V937
  • Western blot analysis of FKBP12 on different cells lysates using anti-FKBP12 antibody at 1/500 dilution.<br />
Positive control: <br />
Line 1: SH-SY5Y           <br />
Line 2:V937
  • ICC staining FKBP12 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining FKBP12 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining FKBP12 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of FKBP12 on different cells lysates using anti-FKBP12 antibody at 1/500 dilution.
Positive control:
Line 1: SH-SY5Y
Line 2:V937

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

FKBP12 Recombinant Rabbit Monoclonal Antibody [JM11-09] (ET1703-55)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY5Y, V937, HepG2, A431, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

12 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

FKBP12

SYNONYMS

12 kDa FK506-binding protein antibody; 12 kDa FKBP antibody; Calstabin 1 antibody; FK506 binding protein 1 antibody; FK506 binding protein 12 antibody; FK506 binding protein 1A (12kD) antibody; FK506 binding protein 1A 12kDa antibody; FK506 binding protein 1A antibody; FK506 binding protein T cell 12 kD antibody; FK506 binding protein, T cell, 12 kD antibody; FK506 binding protein12 antibody; FK506-binding protein 1A antibody; FKB1A_HUMAN antibody; FKBP 12 antibody; FKBP 1A antibody; FKBP-12 antibody; FKBP-1A antibody; FKBP1 antibody; FKBP12 antibody; FKBP12 Exip3 antibody; FKBP12C antibody; fkbp1a antibody; Immunophilin FKBP12 antibody; Peptidyl prolyl cis trans isomerase antibody; Peptidyl-prolyl cis-trans isomerase FKBP1A antibody; PKC12 antibody; PKCI2 antibody; PPIase antibody; PPIase FKBP1A antibody; Protein kinase C inhibitor 2 antibody; Rotamase antibody

SEQUENCE SIMILARITIES

Belongs to the FKBP-type PPIase family. FKBP1 subfamily.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases which bind to and mediate the effects of immunosuppressive drugs such as Cyclosporin, FK506 and Rapamycin. FKBP12.6, also known as FK506-binding protein 1B, is a 108 amino acid immunophilin belonging to the FKBP-type PPIase family. Subcellularly localized to the cytoplasm, FKBP12.6 binds to RyR in cardiac muscle sarcoplasmic reticulum and possibly plays a unique physiological role in excitation-contraction coupling in cardiac muscle. FKBP12.6 also catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Ubiquitously expressed, FKBP12.6 is found at highest levels in brain and thymus. FKBP12.6 is expressed as two isoforms produced by alternative splicing.