Filamin A Recombinant Rabbit Monoclonal Antibody [SA30-08] (ET1601-3)
Catalog# ET1601-3
Rabbit Monoclonal to Filamin A
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
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Monkey
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A549 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C6 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 281 kDa
Observed band size: 281 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-3) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Filamin A Recombinant Rabbit Monoclonal Antibody [SA30-08] (ET1601-3)
Immunogen
Synthetic peptide within human filamin a aa 2,598-2,647 / 2,647.
Host
Rabbit
Positive Control
HeLa cell lysate, HEK-293 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa, C2C12, C6, human uterus tissue, mouse uterus tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA30-08
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:200-1:500
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IF-Tissue
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1:50
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IHC-P
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1:100-1:400
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 281 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Filamin A
SYNONYMS
ABP 280 antibody;ABP-280 antibody;Actin-binding protein 280 antibody;Alpha filamin antibody;Alpha-filamin antibody;APBX antibody;CSBS antibody;CVD1 antibody;Endothelial actin binding protein antibody;Endothelial actin-binding protein antibody;Filamin 1 antibody;Filamin A alpha antibody;Filamin A antibody;Filamin-1 antibody;Filamin-A antibody;FLN antibody;FLN-A antibody;FLN1 antibody;FLNA antibody;FLNA_HUMAN antibody;FMD antibody;MNS antibody;NHBP antibody;Non muscle filamin antibody;Non-muscle filamin antibody;OPD antibody;OPD1 antibody;OPD2 antibody;XLVD antibody;XMVD antibody
SEQUENCE SIMILARITIES
Belongs to the filamin family.
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-2152 is negatively regulated by the autoinhibited conformation of filamin repeats 19-21. Ligand binding induces a conformational switch triggering phosphorylation at Ser-2152 by PKA.; Phosphorylation extent changes in response to cell activation.; Polyubiquitination in the CH1 domain by a SCF-like complex containing ASB2 leads to proteasomal degradation. Prior dissociation from actin may be required to expose the target lysines. Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Actin-binding protein, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed filamin that regulates the reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and secondary messengers. At least 31 disease-causing mutations in this gene have been discovered.
CITATIONS
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Wang Xiaolong,et al
Lactobacillus-Polydopamine System for Targeted Drug Delivery in Overactive Bladder: Evidence from Bladder Cell Spheroids, Rat Models, and Urinary Microbiome Profiling
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IF
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Human
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Citation
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Filamin A is overexpressed in non-alcoholic steatohepatitis and contributes to the progression of inflammation and fibrosis
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WB
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Mouse
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Citation
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