Lane 1: MCF-7 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lane 4: NIH/3T3 cell lysate
Recombinant Rabbit monoclonal primary
Filamin A Recombinant Rabbit Monoclonal Antibody [SA30-08] (ET1601-3)
Synthetic peptide within c-terminal human filamin a.
MCF-7 cell lysate, Jurkat cell lysate, Hela cell lysate, NIH/3T3 cell lysate, Hela, AGS, HUVEC, human uterus tissue, mouse uterus tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
ABP 280 antibody; ABP-280 antibody; Actin-binding protein 280 antibody; Alpha filamin antibody; Alpha-filamin antibody; APBX antibody; CSBS antibody; CVD1 antibody; Endothelial actin binding protein antibody; Endothelial actin-binding protein antibody; Filamin 1 antibody; Filamin A alpha antibody; Filamin A antibody; Filamin-1 antibody; Filamin-A antibody; FLN antibody; FLN-A antibody; FLN1 antibody; FLNA antibody; FLNA_HUMAN antibody; FMD antibody; MNS antibody; NHBP antibody; Non muscle filamin antibody; Non-muscle filamin antibody; OPD antibody; OPD1 antibody; OPD2 antibody; XLVD antibody; XMVD antibody
Belongs to the filamin family.
Phosphorylation at Ser-2152 is negatively regulated by the autoinhibited conformation of filamin repeats 19-21. Ligand binding induces a conformational switch triggering phosphorylation at Ser-2152 by PKA.; Phosphorylation extent changes in response to cell activation.; Polyubiquitination in the CH1 domain by a SCF-like complex containing ASB2 leads to proteasomal degradation. Prior dissociation from actin may be required to expose the target lysines. Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome.
Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.