PRODUCT CODE: ER1802-95

Fibulin 5 Rabbit Polyclonal Antibody (ER1802-95)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.
  • Western blot analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.
  • ICC staining Fibulin 5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fibulin 5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fibulin 5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SiHa cells with Fibulin 5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Fibulin 5 Rabbit Polyclonal Antibody (ER1802-95)

Immunogen

Recombinant protein within human fibulin aa 203-378 / 448.

Host

Rabbit

Positive Control

Human skin tissue, JAR, SiHa, SKOV-3, rat testis tissue, mouse ovary tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Fibulin 5

SYNONYMS

ADCL2 antibody; ARCL1A antibody; ARMD3 antibody; Dance antibody; Developmental arteries and neural crest EGF like protein antibody; Developmental arteries and neural crest EGF-like protein antibody; EVEC antibody; Fbln5 antibody; FBLN5_HUMAN antibody; FIBL 5 antibody; FIBL-5 antibody; Fibulin-5 antibody; FLJ90059 antibody; UP50 antibody; Urine p50 protein antibody

SEQUENCE SIMILARITIES

Belongs to the fibulin family.

TISSUE SPECIFICITY

Expressed in skin fibroblasts (at protein level)(PubMed:17035250). Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Extracellular matrix. Secreted.

FUNCTION

Fibulin proteins contribute to normal development of elastic fiber systems in various types of organs that require elasticity, such as vasculature, lung and skin. Fibulin-5 (EVEC, UP50, DANCE) is an integrin-binding extracellular matrix protein that mediates endothelial cell adhesion. Fibulin-5 is also a calcium-dependent elastin-binding protein that scaffolds cells to elastic fibers, thereby preventing elastinopathy in the skin, lung, and vasculature. The Arg-Gly-Asp (RGD) motif in Fibulin-5 interacts with cell surface integrins avb3, avb5 and a9b1, serves as an anchorage for elastic fibers to cells, and promotes organization of elastic fibers. The human Fibulin-5 gene maps to chromosome 14q32.12 and encodes a 488 amino acid protein.