PRODUCT CODE: ET7108-46

Fibrinogen gamma chain Recombinant Rabbit Monoclonal Antibody [JG37-18] (ET7108-46)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Fibrinogen gamma chain on human plasma using anti-Fibrinogen gamma chain at 1/5,000 dilution.
  • Western blot analysis of Fibrinogen gamma chain on human plasma using anti-Fibrinogen gamma chain at 1/5,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Fibrinogen gamma chain antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Fibrinogen gamma chain antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Fibrinogen gamma chain on human plasma using anti-Fibrinogen gamma chain at 1/5,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Fibrinogen gamma chain Recombinant Rabbit Monoclonal Antibody [JG37-18] (ET7108-46)

Immunogen

Recombinant protein corresponding to human fibrinogen gamma 50-250aa.

Host

Rabbit

Positive Control

Human plasma, human colon tissue, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG37-18

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000-1:10,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Fibrinogen gamma chain

SYNONYMS

FGG antibody; FIBG_HUMAN antibody; Fibrinogen gamma chain antibody; Fibrinogen gamma polypeptide antibody; fibrinogen gamma-b chain antibody

TISSUE SPECIFICITY

Detected in blood plasma (at protein level).

POST-TRANSLATIONAL MODIFICATION

Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.; Sulfation of C-terminal tyrosines increases affinity for thrombin.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.