PRODUCT CODE: M1510-13

Fibrinogen Mouse Monoclonal Antibody [1-A7] (M1510-13)

Applications

  • WB

  • IHC-P

  • ELISA

REACTIVITY

  • Human

Applications

  • WB

  • IHC-P

  • ELISA

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Fibrinogen Mouse Monoclonal Antibody [1-A7] (M1510-13)

Immunogen

Native protein

Host

Mouse

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1-A7

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

39 kDa

Isotype

IgA

TARGET

UNIPROT #

PROTEIN NAME

Fibrinogen

TISSUE SPECIFICITY

Detected in blood plasma (at protein level).

POST-TRANSLATIONAL MODIFICATION

The alpha chain is normally not N-glycosylated. It is well known that heterologous expression of isolated domains can lead to adventitious protein modifications. Besides, glycosylation at Asn-686 is supported by large-scale glycoproteomics studies (PubMed:16335952 and PubMed:19159218), but the evidence is still quite tenuous. Most likely, Asn-686 is not glycosylated in the healthy human body, or only with low efficiency.; O-glycosylated.; Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.; About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.; Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.; Phosphorylated by FAM20C in the extracellular medium.