Fibrillarin Rabbit Polyclonal Antibody (ER1802-81)
Catalog# ER1802-81
Rabbit Polyclonal to Fibrillarin
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WB
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FC
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IHC-P
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IF-Tissue
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Human
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Mouse
Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH-3T3 cell lysate
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Fibrillarin Rabbit Polyclonal Antibody (ER1802-81)
Immunogen
Synthetic peptide within human fibrillarin aa 272-321 / 321.
Host
Rabbit
Positive Control
Hela cell lysate, HepG2 cell lysate, 293T cell lysate, NIH-3T3 cell lysate, HL-60, human tonsil tissue, mouse liver tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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FC
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1:50-1:100
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IHC-P
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1:200-1:1,000
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IF-Tissue
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1:50-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 34 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Fibrillarin
SYNONYMS
34 kD nucleolar scleroderma antigen antibody;34 kDa nucleolar scleroderma antigen antibody;Fbl antibody;FBRL_HUMAN antibody;FIB antibody;FIB1 antibody;FLRN antibody;Histone-glutamine methyltransferase antibody;Nop1p antibody;RNA U3 small nucleolar interacting protein 1 antibody;RNU3IP1 antibody;rRNA 2' O methyltransferase fibrillarin antibody;rRNA 2'-O-methyltransferase fibrillarin antibody
SEQUENCE SIMILARITIES
Belongs to the methyltransferase superfamily. Fibrillarin family.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36.; By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).; Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation. Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me).
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Fibrillarin is a widely occurring, basic, nonhistone protein that is localized exclusively in the fibrillar region of the nucleolus, including both the dense fibrillar and the fibrillar center regions. Fibrillarin is also expressed in HeLa cells, 3T3 cells, and human peripheral blood lymphocytes. In metaphase and anaphase, fibrillarin is found on putative chromosomal nucleolar regions (NORs). During telophase, fibrillarin is an early marker for the site of the newly forming nucleolus. The structure of fibrillarin includes an RNA-binding domain and an RNP consensus sequence, which is consistent with the association of fibrillarin with the U3 small nucleolar RNA. Fibrillarin is involved in processing rRNA transcripts in the nucleolus.
CITATIONS
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Wu, J., Li, X., Gao, Z., Pang, L., Liu, X., Huang, X., Wang, Y., & Wang, Z.
RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis
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IF
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Drosophila
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Citation
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