PRODUCT CODE: ET1703-78

FGFR2 Recombinant Rabbit Monoclonal Antibody [JM10-60] (ET1703-78)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of FGFR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: Jurkat cell lysate
  • Western blot analysis of FGFR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: Jurkat cell lysate
Western blot analysis of FGFR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Jurkat cell lysate

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

FGFR2 Recombinant Rabbit Monoclonal Antibody [JM10-60] (ET1703-78)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-60

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

145 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

FGFR2

SYNONYMS

bacteria-expressed kinase antibody; BBDS antibody; BEK antibody; BEK fibroblast growth factor receptor antibody; BFR1 antibody; CD332 antibody; CD332 antigen antibody; CEK3 antibody; CFD1 antibody; Craniofacial dysostosis 1 antibody; ECT1 antibody; FGF receptor antibody; FGFR 2 antibody; FGFR-2 antibody; Fgfr2 antibody; FGFR2_HUMAN antibody; Fibroblast growth factor receptor 2 antibody; Hydroxyaryl protein kinase antibody; Jackson Weiss syndrome antibody; JWS antibody; K SAM antibody; K-sam antibody; Keratinocyte growth factor receptor 2 antibody; Keratinocyte growth factor receptor antibody; KGFR antibody; KSAM antibody; protein tyrosine kinase, receptor like 14 antibody; soluble FGFR4 variant 4 antibody; TK14 antibody; TK25 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on several tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-769 is essential for interaction with PLCG1.; N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus.; Ubiquitinated. FGFR2 is rapidly ubiquitinated after autophosphorylation, leading to internalization and degradation. Subject to degradation both in lysosomes and by the proteasome.

SUBCELLULAR LOCATION

Cell membrane, Golgi apparatus, Cytoplasmic vesicle.

FUNCTION

Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Like other growth factors, FGFs act by binding and activating specific cell surface receptors. These include the Flg receptor or FGFR-1, the Bek receptor (or FGFR-2), FGFR-3, FGFR-4, FGFR-5 and FGFR-6. These receptors usually contain an extracellular ligand-binding region containing three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The gene encoding human Bek (also designated K-sam) maps to chromosome 10q26.13 and is alternatively spliced to produce several isoforms. Heterogeneous mutations in Bek are associated with a range of craniosynostosis syndromes including Pfeiffer syndrome, Crouzon syndrome, Jackson-Weiss syndrome and Apert syndrome..