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PRODUCT CODE: ER2001-44

FCGRT Rabbit Polyclonal Antibody (ER2001-44)

Applications

  • WB

REACTIVITY

  • Human

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Western blot analysis of FCGRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Human brain tissue lysate<br /> Lane 2: HepG2 cell lysate
  • Western blot analysis of FCGRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Human brain tissue lysate<br /> Lane 2: HepG2 cell lysate
Western blot analysis of FCGRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human brain tissue lysate
Lane 2: HepG2 cell lysate

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

FCGRT Rabbit Polyclonal Antibody (ER2001-44)

Immunogen

Synthetic peptide within human fcgrt aa 20-70.

Host

Rabbit

Positive Control

Human brain tissue lysate, HepG2 cell lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size: 40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

P55899

PROTEIN NAME

FCGRT

SYNONYMS

Alpha chain antibody; Fc fragment of IgG, receptor transporter, alpha antibody; FCGRN_HUMAN antibody; FCGRT antibody; FcRn alpha chain antibody; FcRn antibody; FCRN, alpha chain antibody; IgG Fc fragment receptor transporter alpha chain antibody; IgG Gc receptor antibody; IgG receptor FcRn large subunit p51 antibody; IgG receptor FcRn large subunit p51 precursor antibody; Immunoglobulin receptor, intestinal, heavy chain antibody; Neonatal Fc receptor antibody

SUBCELLULAR LOCATION

Cell membrane, Endosome, Membrane.

FUNCTION

Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk . IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation . In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB .