Positive control:
Lane 1: Mouse spleen tissue lysate
Lane 1: Rat lung tissue lysate
Lane 2: HepG2 cell lysate
Applications
-
WB
-
ICC
-
FC
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
FBXW7 Rabbit Polyclonal Antibody (ER1706-78)
Immunogen
Recombinant protein within human fbxw7 aa 84-327.
Host
Rabbit
Positive Control
Mouse spleen tissue lysate, rat lung tissue lysate, HepG2 cell lysate, HUVEC, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
70 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
ICC
-
1:500-1:2,000
-
FC
-
1:50-1:100
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
FBXW7
SYNONYMS
AGO antibody; Archipelago antibody; Archipelago homolog antibody; Archipelago, Drosophila, homolog of antibody; CDC4 antibody; CDC4, S.cerevisiae, homolog of antibody; DKFZp686F23254 antibody; F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody; F box and WD 40 domain protein 7 antibody; F box and WD repeat domain containing 7 antibody; F box protein FBW7 antibody; F box protein FBX30 antibody; F box protein SEL 10 antibody; F box protein SEL10 antibody; F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase antibody; F-box and WD-40 domain-containing protein 7 antibody; F-box protein FBX30 antibody; F-box/WD repeat-containing protein 7 antibody; FBW6 antibody; FBW7 antibody; FBX30 antibody; FBXO30 antibody; FBXW6 antibody; FBXW7 antibody; FBXW7_HUMAN antibody; FLJ16457 antibody; hAgo antibody; hCdc4 antibody; Homolog of C elegans sel 10 antibody; Homolog of C.elegans sel10 antibody; SEL 10 antibody; SEL-10 antibody; SEL10 antibody
TISSUE SPECIFICITY
Isoform 1 is widely expressed. Isoform 3 is expressed in brain.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Thr-205 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation.; Ubiquitinated: autoubiquitinates following phosphorylation at Thr-205 and subsequent interaction with PIN1. Ubiquitination leads to its proteasomal degradation.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation