PRODUCT CODE: ER1706-78

FBXW7 Rabbit Polyclonal Antibody (ER1706-78)

Applications

  • WB

  • ICC

  • FC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of FBXW7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1706-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse spleen tissue lysate<br />
Lane 1: Rat lung tissue lysate<br />
Lane 2: HepG2 cell lysate
  • Western blot analysis of FBXW7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1706-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse spleen tissue lysate<br />
Lane 1: Rat lung tissue lysate<br />
Lane 2: HepG2 cell lysate
  • ICC staining of FBXW7 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of FBXW7 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Flow cytometric analysis of FBXW7 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1706-78, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of FBXW7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1706-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse spleen tissue lysate
Lane 1: Rat lung tissue lysate
Lane 2: HepG2 cell lysate

Applications

  • WB

  • ICC

  • FC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

FBXW7 Rabbit Polyclonal Antibody (ER1706-78)

Immunogen

Recombinant protein within human fbxw7 aa 84-327.

Host

Rabbit

Positive Control

Mouse spleen tissue lysate, rat lung tissue lysate, HepG2 cell lysate, HUVEC, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

FBXW7

SYNONYMS

AGO antibody; Archipelago antibody; Archipelago homolog antibody; Archipelago, Drosophila, homolog of antibody; CDC4 antibody; CDC4, S.cerevisiae, homolog of antibody; DKFZp686F23254 antibody; F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody; F box and WD 40 domain protein 7 antibody; F box and WD repeat domain containing 7 antibody; F box protein FBW7 antibody; F box protein FBX30 antibody; F box protein SEL 10 antibody; F box protein SEL10 antibody; F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase antibody; F-box and WD-40 domain-containing protein 7 antibody; F-box protein FBX30 antibody; F-box/WD repeat-containing protein 7 antibody; FBW6 antibody; FBW7 antibody; FBX30 antibody; FBXO30 antibody; FBXW6 antibody; FBXW7 antibody; FBXW7_HUMAN antibody; FLJ16457 antibody; hAgo antibody; hCdc4 antibody; Homolog of C elegans sel 10 antibody; Homolog of C.elegans sel10 antibody; SEL 10 antibody; SEL-10 antibody; SEL10 antibody

TISSUE SPECIFICITY

Isoform 1 is widely expressed. Isoform 3 is expressed in brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-205 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation.; Ubiquitinated: autoubiquitinates following phosphorylation at Thr-205 and subsequent interaction with PIN1. Ubiquitination leads to its proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation