Fbx32 Recombinant Rabbit Monoclonal Antibody [JE41-27] (ET7109-25)
Catalog# ET7109-25
Rabbit Monoclonal to Fbx32
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WB
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-25) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Fbx32 Recombinant Rabbit Monoclonal Antibody [JE41-27] (ET7109-25)
Immunogen
Recombinant protein within human fbx32 aa 1-160 / 355.
Host
Rabbit
Positive Control
Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, A431, L6.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE41-27
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 41 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Fbx32
SYNONYMS
4833442G10Rik antibody;AI430017 antibody;Atrogin 1 antibody;Atrogin-1 antibody;ATROGIN1 antibody;Atrophy gene 1 antibody;F box only protein 32 antibody;F-box only protein 32 antibody;F-box protein 32 antibody;FBX32_HUMAN antibody;fbxo25 antibody;FBXO32 antibody;FLJ32424 antibody;MAFbx antibody;MGC108443 antibody;MGC137646 antibody;MGC33610 antibody;Muscle atrophy F box antibody;Muscle atrophy F box protein antibody;Muscle atrophy F-box protein antibody
TISSUE SPECIFICITY
Specifically expressed in cardiac and skeletal muscle.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
Muscle atrophy F-box (MAFbx) is an E3 ubiquitin ligase that initiates ATP-dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. MAFbx transcript is abundant in cardiac and skeletal muscle undergoing atrophy. MAFbx -/- mice are resistant to muscle atrophy. MAFbx is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. It interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. MAFbx is specifically expressed in cardiac and skeletal muscle.
CITATIONS
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Punicalagin protects against impaired skeletal muscle function in high-fat-diet-induced obese mice by regulating TET2
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WB
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Mouse
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Citation
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Li Caixia,et al
Fucoidan ameliorates diabetic skeletal muscle atrophy through PI3K/Akt pathway
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WB
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Rat
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Citation
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Chen J, Chen XY, Cong XX, et al
CELLULAR SENESCENCE IMPLICATED IN SEPSIS-INDUCED MUSCLE WEAKNESS AND AMELIORATED WITH METFORMIN
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WB
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Mouse
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Citation
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