Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Fbx32 on mouse skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST in PBS for 1 hour at room temperature. The primary antibody (ET7109-25, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 41 kDa
Observed band size: 41 kDa
4833442G10Rik antibody;AI430017 antibody;Atrogin 1 antibody;Atrogin-1 antibody;ATROGIN1 antibody;Atrophy gene 1 antibody;F box only protein 32 antibody;F-box only protein 32 antibody;F-box protein 32 antibody;FBX32_HUMAN antibody;fbxo25 antibody;FBXO32 antibody;FLJ32424 antibody;MAFbx antibody;MGC108443 antibody;MGC137646 antibody;MGC33610 antibody;Muscle atrophy F box antibody;Muscle atrophy F box protein antibody;Muscle atrophy F-box protein antibody
Specifically expressed in cardiac and skeletal muscle.
Muscle atrophy F-box (MAFbx) is an E3 ubiquitin ligase that initiates ATP-dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. MAFbx transcript is abundant in cardiac and skeletal muscle undergoing atrophy. MAFbx -/- mice are resistant to muscle atrophy. MAFbx is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. It interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. MAFbx is specifically expressed in cardiac and skeletal muscle.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.