Fascin Rabbit Polyclonal Antibody (ER1901-71)
Catalog# ER1901-71
Rabbit Polyclonal to Fascin
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Fascin KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-71) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Fascin Rabbit Polyclonal Antibody (ER1901-71)
Immunogen
Recombinant protein within human fascin aa 1-140 / 493.
Host
Rabbit
Positive Control
SH-SY5Y, rat brain tissue, human tonsil tissue, human kidney tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB:1:1,000
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IHC-P:1:50-1:200
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FC:1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 54 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Fascin
SYNONYMS
55 kDa actin bundling protein antibody;55 kDa actin-bundling protein antibody;Actin bundling protein antibody;actin bundling protein, 55-KD antibody;FAN 1 antibody;FAN1 antibody;Fascin 1 antibody;Fascin actin bundling protein 1 antibody;Fascin antibody;Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody;Fascin homolog 1 antibody;Fascin, sea urchin, homolog of, 1 antibody;Fascin1 antibody;FLJ38511 antibody;FSCN 1 antibody;FSCN1 antibody;FSCN1_HUMAN antibody;HSN antibody;p55 antibody;Singed (Drosophila) like (sea urchin fascin homolog like) antibody;Singed drosophila homolog like antibody;Singed like (fascin homolog sea urchin) (Drosophila) antibody;Singed like (fascin homolog sea urchin) antibody;Singed like protein antibody;Singed, drosophila, homolog of antibody;Singed-like protein antibody;SNL antibody;Strongylocentrotus purpuratus antibody
SEQUENCE SIMILARITIES
Belongs to the fascin family.
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-39 inhibits actin-binding. Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF.
SUBCELLULAR LOCATION
Cytoskeleton. Cytosol.
FUNCTION
Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF. Cell adhesion to extracellular matrix is an important physiological stimulus for organization of the Actin-based cytoskeleton. Adhesion to the matrix glycoprotein Thrombospondin 1 triggers the sustained formation of F-Actin microspikes that contain the Actin-bundling protein Fascin. These structures are also implicated in cell migration, which may be an important function of Thrombospondin 1 in tissue remodelling and wound repair. Fascin bundles Actin microfilaments within dynamic cellular structures such as microspikes, stress fibers and membrane ruffles. Fascin could serve as a prognostic factor for abnormal ovarian epithelial pathology and could be a novel target for the treatment of ovarian cancer. Fascin, an Actin-bundling protein, identifies dendritic cells in the blood and in tissues.
CITATIONS
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Chu Yajie,et al
Exposure to chlorpyrifos interferes with intercellular communication in cumulus-oocyte complexes during porcine oocyte maturation
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WB
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Pig
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Citation
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