FAM134B Recombinant Rabbit Monoclonal Antibody [PSH01-94] (HA721752)
Catalog# HA721752
Rabbit Monoclonal to FAM134B
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WB
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: HEK-293 cell lysate (30 µg/Lane)
Lane 2: U-2 OS cell lysate (30 µg/Lane)
Lane 3: MCF7 cell lysate (30 µg/Lane)
Lane 4: HeLa cell lysate (30 µg/Lane)
Lane 5: HepG2 cell lysate (30 µg/Lane)
Lane 6: TT cell lysate (30 µg/Lane)
Lane 7: Jurkat cell lysate (30 µg/Lane)
Lane 8: NIH/3T3 cell lysate (30 µg/Lane)
Lane 9: C2C12 cell lysate (30 µg/Lane)
Lane 10: PC-12 cell lysate (30 µg/Lane)
Lane 11: C6 cell lysate (30 µg/Lane)
Lane 12: Mouse liver tissue lysate (30 µg/Lane)
Lane 13: Rat liver tissue lysate (30 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 70 kDa
Exposure time: 6 minutes 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
FAM134B Recombinant Rabbit Monoclonal Antibody [PSH01-94] (HA721752)
Immunogen
Recombinant protein within human fam134b aa 318-497 / 497.
Host
Rabbit
Positive Control
HEK-293 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, HeLa cell lysate, HepG2 cell lysate, TT cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH01-94
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 55 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
FAM134B
SYNONYMS
Reticulophagy regulator 1;RETREG1;Reticulophagy receptor 1;FAM134B;JK1
SUBCELLULAR LOCATION
olgi apparatus, cis-Golgi network membrane, Endoplasmic reticulum membrane.
FUNCTION
Endoplasmic reticulum (ER)-anchored autophagy regulator which mediates ER delivery into lysosomes through sequestration into autophagosomes . Promotes membrane remodeling and ER scission via its membrane bending capacity and targets the fragments into autophagosomes via interaction with ATG8 family proteins . Active under basal conditions. Required for collagen quality control in a LIR motif-dependent manner (By similarity). Required for long-term survival of nociceptive and autonomic ganglion neurons.
CITATIONS
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Wei Xiaohong,et al
Overexpression of long noncoding RNA DUXAP8 inhibits ER-phagy through activating AKT/mTOR signaling and contributes to preeclampsia
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WB
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Rat
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Citation
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