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PRODUCT CODE: ER1902-34

Factor XIIIa Rabbit Polyclonal Antibody (ER1902-34)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of Factor XIIIa on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: human placenta tissue lysate<br /> Lane 2: human liver tissue lysate
  • Western blot analysis of Factor XIIIa on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: human placenta tissue lysate<br /> Lane 2: human liver tissue lysate
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Factor XIIIa antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-34, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Factor XIIIa was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-34, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green).
Western blot analysis of Factor XIIIa on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: human placenta tissue lysate
Lane 2: human liver tissue lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Factor XIIIa Rabbit Polyclonal Antibody (ER1902-34)

Immunogen

Recombinant protein within human factor xiiia aa 259-463 / 732.

Host

Rabbit

Positive Control

Human placenta tissue lysate, human liver tissue lysate, human placenta tissue, Hela.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size 83 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P00488

PROTEIN NAME

Factor XIIIa

SYNONYMS

bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody; Coagulation factor XIII A chain antibody; Coagulation factor XIII A1 polypeptide antibody; Coagulation factor XIII A1 subunit antibody; Coagulation factor XIII, A polypeptide antibody; Coagulation factor XIIIa antibody; F13A antibody; F13A_HUMAN antibody; F13a1 antibody; Factor XIIIA antibody; Fibrin stabilizing factor, A subunit antibody; Fibrinoligase antibody; FSF, A subunit antibody; Protein glutamine gamma glutamyltransferase A chain antibody; Protein-glutamine gamma-glutamyltransferase A chain antibody; TGase antibody; Transglutaminase A chain antibody; Transglutaminase, plasma antibody; Transglutaminase. plasma antibody

SEQUENCE SIMILARITIES

Belongs to the transglutaminase superfamily. Transglutaminase family.

POST-TRANSLATIONAL MODIFICATION

The activation peptide is released by thrombin.

SUBCELLULAR LOCATION

Cytoplasm, Secreted.

FUNCTION

This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.