Positive control:
Lane 1: human placenta tissue lysate
Lane 2: human liver tissue lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Factor XIIIa Rabbit Polyclonal Antibody (ER1902-34)
Immunogen
Recombinant protein within human factor xiiia aa 259-463 / 732.
Host
Rabbit
Positive Control
Human placenta tissue lysate, human liver tissue lysate, human placenta tissue, Hela.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
Predicted band size 83 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Factor XIIIa
SYNONYMS
bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody; Coagulation factor XIII A chain antibody; Coagulation factor XIII A1 polypeptide antibody; Coagulation factor XIII A1 subunit antibody; Coagulation factor XIII, A polypeptide antibody; Coagulation factor XIIIa antibody; F13A antibody; F13A_HUMAN antibody; F13a1 antibody; Factor XIIIA antibody; Fibrin stabilizing factor, A subunit antibody; Fibrinoligase antibody; FSF, A subunit antibody; Protein glutamine gamma glutamyltransferase A chain antibody; Protein-glutamine gamma-glutamyltransferase A chain antibody; TGase antibody; Transglutaminase A chain antibody; Transglutaminase, plasma antibody; Transglutaminase. plasma antibody
SEQUENCE SIMILARITIES
Belongs to the transglutaminase superfamily. Transglutaminase family.
POST-TRANSLATIONAL MODIFICATION
The activation peptide is released by thrombin.
SUBCELLULAR LOCATION
Cytoplasm, Secreted.
FUNCTION
This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.