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FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99]

Usd: 385

Specification

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Catalog# ET1703-98

FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99]

Application

  • WB

  • IF-Cell

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human FABP4 aa 60-100.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-Fr

Molecular Weight

Predicted band size: 15 kDa

Positive Control

Mouse white adipose tissue lysate, Mouse brown adipose tissue lysate, Mouse kidney tissue lysate, Rat white adipose tissue lysate, Rat brown adipose tissue lysate, Rat kidney tissue lysate, HAEC.

Conjugation

unconjugated

Clone Number

JM10-99

RRID

AB_3070452

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:100-1:500

  • IHC-Fr

  • 1:100

Target

Function

Fatty acid-binding proteins, designated FABPs, are a family of homologous, cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport, and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epidermis (E-FABP, also designated psoriasis-associated FABP or PA-FABP), muscle and heart (H-FABP, also designated mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP), myelin (M-FABP) and testis (T-FABP). The human A-FABP gene is organized into 4 exons, maps to chromosome 8q21, and encodes a 132-amino acid protein. A-FABP protein comprises approximately 1% of the total cytosolic protein in human adipose tissue.

Background References

1. Roberts JN et al. Dynamic Surfaces for the Study of Mesenchymal Stem Cell Growth through Adhesion Regulation. ACS Nano 10:6667-79 (2016).

2. Ohyama Y et al. Differential expression of fatty acid-binding proteins and pathological implications in the progression of tongue carcinoma. Mol Clin Oncol 2:19-25 (2014).

Sequence Similarity

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Subcellular Location

Nucleus. Cytoplasm.

UNIPROT

SwissProt: P15090 Human

SwissProt: P04117 Mouse

SwissProt: P70623 Rat

Synonyms

3T3-L1 lipid-binding protein antibody

422/aP2 antibody

A-FABP antibody

adipocyte antibody

Adipocyte lipid binding protein antibody

Adipocyte lipid-binding protein antibody

Adipocyte protein AP2 antibody

Adipocyte-type fatty acid-binding protein antibody

AFABP antibody

ALBP antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of FABP4 on different lysates with Rabbit anti-FABP4 antibody (<a href="/products/ET1703-98" style="font-weight: bold;text-decoration: underline;">ET1703-98</a>) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.<br /><br />Lane 1: Mouse white adipose tissue lysate<br />Lane 2: Mouse brown adipose tissue lysate<br />Lane 3: Mouse kidney tissue lysate (negative)<br />Lane 4: Rat white adipose tissue lysate<br />Lane 5: Rat brown adipose tissue lysate<br />Lane 6: Rat kidney tissue lysate (negative)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 15 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: 3 minutes 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-98" style="font-weight: bold;text-decoration: underline;">ET1703-98</a>) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of FABP4 on different lysates with Rabbit anti-FABP4 antibody (ET1703-98) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.

    Lane 1: Mouse white adipose tissue lysate
    Lane 2: Mouse brown adipose tissue lysate
    Lane 3: Mouse kidney tissue lysate (negative)
    Lane 4: Rat white adipose tissue lysate
    Lane 5: Rat brown adipose tissue lysate
    Lane 6: Rat kidney tissue lysate (negative)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

    Exposure time: 3 minutes 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-98) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HAEC cells labeling FABP4 with Rabbit anti-FABP4 antibody (<a href="/products/ET1703-98" style="font-weight: bold;text-decoration: underline;">ET1703-98</a>) at 1/500 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FABP4 antibody (<a href="/products/ET1703-98" style="font-weight: bold;text-decoration: underline;">ET1703-98</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.n.

    Immunocytochemistry analysis of HAEC cells labeling FABP4 with Rabbit anti-FABP4 antibody (ET1703-98) at 1/500 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FABP4 antibody (ET1703-98) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.n.

  • Immunofluorescence analysis of frozen mouse adipose tissue labeling FABP4 with Rabbit anti-FABP4 antibody (<a href="/products/ET1703-98" style="font-weight: bold;text-decoration: underline;">ET1703-98</a>).<br /><br />The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1703-98" style="font-weight: bold;text-decoration: underline;">ET1703-98</a>, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor&reg; 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

    Immunofluorescence analysis of frozen mouse adipose tissue labeling FABP4 with Rabbit anti-FABP4 antibody (ET1703-98).

    The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-98, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99] - BSA and Azide free

Application: WB,IF-Cell,IHC-Fr

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated