FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99] (ET1703-98)
Catalog# ET1703-98
Rabbit Monoclonal to FABP4
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WB
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: Mouse white adipose tissue lysate
Lane 2: Mouse brown adipose tissue lysate
Lane 3: Mouse kidney tissue lysate (negative)
Lane 4: Rat white adipose tissue lysate
Lane 5: Rat brown adipose tissue lysate
Lane 6: Rat kidney tissue lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-98) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99] (ET1703-98)
Immunogen
Synthetic peptide within human fabp4 aa 60-100.
Host
Rabbit
Positive Control
Mouse white adipose tissue lysate, mouse brown adipose tissue lysate, rat white adipose tissue lysate, rat brown adipose tissue lysate, HAEC, MCF-7, A549, PMVEC.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM10-99
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:100-1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 15 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
FABP4
SYNONYMS
3T3-L1 lipid-binding protein antibody;422/aP2 antibody;A-FABP antibody;adipocyte antibody;Adipocyte lipid binding protein antibody;Adipocyte lipid-binding protein antibody;Adipocyte protein AP2 antibody;Adipocyte-type fatty acid-binding protein antibody;AFABP antibody;ALBP antibody;ALBP/Ap2 antibody;aP2 antibody;Epididymis secretory protein Li 104 antibody;FABP antibody;FABP4 antibody;FABP4_HUMAN antibody;Fatty acid binding protein 4 adipocyte antibody;Fatty acid binding protein 4 antibody;Fatty acid binding protein adipocyte antibody;Fatty acid-binding protein 4 antibody;Fatty acid-binding protein antibody;HEL S 104 antibody;Lbpl antibody;Myelin P2 protein homolog antibody;P15 antibody;P2 adipocyte protein antibody;Protein 422 antibody
SEQUENCE SIMILARITIES
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
SUBCELLULAR LOCATION
Nucleus. Cytoplasm.
FUNCTION
Fatty acid-binding proteins, designated FABPs, are a family of homologous, cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport, and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epidermis (E-FABP, also designated psoriasis-associated FABP or PA-FABP), muscle and heart (H-FABP, also designated mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP), myelin (M-FABP) and testis (T-FABP). The human A-FABP gene is organized into 4 exons, maps to chromosome 8q21, and encodes a 132-amino acid protein. A-FABP protein comprises approximately 1% of the total cytosolic protein in human adipose tissue.
CITATIONS
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Chen, W., Xu, Z., You, W., Zhou, Y., Wang, L., Huang, Y., & Shan, T.
Cold exposure alters lipid metabolism of skeletal muscle through HIF-1α-induced mitophagy
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WB
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Mouse
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Citation
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GADD45A regulates subcutaneous fat deposition and lipid metabolism by interacting with Stat1
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WB
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Mouse
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Citation
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Activation of the PERK-CHOP signaling pathway during endoplasmic reticulum stress contributes to olanzapine-induced dyslipidemia
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WB
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Mouse
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Citation
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Liu, Y., Chen, Y., Wang, Y., Jiang, S., Lin, W., Wu, Y., Li, Q., Guo, Y., Liu, W., & Yuan, Q.
DNA demethylase ALKBH1 promotes adipogenic differentiation via regulation of HIF-1 signaling
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WB
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Human
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Citation
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-
Li, L., Tao, S., Guo, F., Liu, J., Huang, R., Tan, Z., Zeng, X., Ma, L., & Fu, P.
Genetic and pharmacological inhibition of fatty acid-binding protein 4 alleviated inflammation and early fibrosis after toxin induced kidney injury. International immunopharmacology, 96, 107760. Advance online publication.
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Liang Ma;Ping Fu
Inhibition of Fatty Acid–Binding Protein 4 Attenuated Kidney Fibrosis by Mediating Macrophage-to-Myofibroblast Transition
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WB
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IHC
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IF
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human
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Citation
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-
Tizhong Shan
GADD45α drives brown adipose tissue formation through upregulating PPARγ in mice
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WB
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mice
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Citation
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-
Liang Ma,Ping Fu
Pharmacological and genetic inhibition of fatty acid‐binding protein 4 alleviated cisplatin‐induced acute kidney injury
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WB
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IF
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mice
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Citation
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-
Ren, Q., Wang, B., Guo, F., Huang, R., Tan, Z., Ma, L., & Fu, P.
Natural Flavonoid Pectolinarigenin Alleviated Hyperuricemic Nephropathy via Suppressing TGFβ/SMAD3 and JAK2/STAT3 Signaling Pathways
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WB
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IHC
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Mouse
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Citation
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Zhang, P., Tian, L., Bao, J., Li, S., Li, A., Wen, Y., Wang, J., & Jie, Y.
Isotretinoin Impairs the Secretory Function of Meibomian Gland Via the PPARγ Signaling Pathway
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WB
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Rat
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Citation
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