F4/80 Recombinant Rabbit Monoclonal Antibody [PSH01-87] (HA721745)
Catalog# HA721745
Rabbit Monoclonal to F4/80
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WB
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IHC-P
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IF-Tissue
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IHC-Fr
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Mouse
Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721745) at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane)
Lane 2: L-929 cell lysate (no heat) (negative) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 102 kDa
Observed band size: 160 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721745) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
F4/80 Recombinant Rabbit Monoclonal Antibody [PSH01-87] (HA721745)
Immunogen
Recombinant protein within mouse f4/80 aa 1-650 / 931.
Host
Rabbit
Positive Control
RAW264.7 cell lysate, mouse liver tissue, mouse spleen tissue, mouse lung tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH01-87
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:5,000
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IF-Tissue
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1:500
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IHC-Fr
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 102 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
F4/80
SYNONYMS
ADGRE1 antibody;Adhesion G protein coupled receptor E1 antibody;Adhesion G protein-coupled receptor E1 antibody;AGRE1_HUMAN antibody;Cell surface glycoprotein EMR1 antibody;Cell surface glycoprotein F4/80 antibody;DD7A5 7 antibody;Egf like module containing mucin like hormone receptor like 1 antibody;Egf like module containing mucin like hormone receptor like sequence 1 antibody;EGF like module receptor 1 antibody;EGF TM7 antibody;EGF-like module receptor 1 antibody;EGF-like module-containing mucin-like hormone receptor-like 1 antibody;EGFTM7 antibody;EMR 1 antibody;EMR1 antibody;EMR1 hormone receptor antibody;Gpf480 antibody;Ly71 antibody;Lymphocyte antigen 71 antibody;TM7LN3 antibody
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. EMR1 is a member of the adhesion GPCR family. Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain. EMR1 expression in human is restricted to eosinophils and is a specific marker for these cells. The murine homolog of EMR1, F4/80, is a well-known and widely used marker of murine macrophage populations. The N-terminal fragment (NTF) of EMR1 contains 4-6 Epidermal Growth Factor-like (EGF-like) domains in human and 4-7 EGF-like domains in the mouse. Utilizing F4/80 knockout mice, Lin et al. showed that F4/80 is not necessary for the development of tissue macrophages but is required for the induction of efferent CD8+ regulatory T cells needed for peripheral tolerance.
CITATIONS
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Wang Yanan,et al
Self-Sulfhydrated, Nitro-Fixed Albumin Nanoparticles as a Potent Therapeutic Agent for the Treatment of Acute Liver Injury
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IF
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Mouse
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Citation
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