Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Recombinant Rabbit monoclonal primary
Ezrin Recombinant Rabbit Monoclonal Antibody [JM10-65] (ET1703-25)
Synthetic peptide within human ezrin aa 350-400.
Hela, Jurkat, A431, human lung tissue, human lung cancer tissue, human liver cancer tissue, human colon cancer tissue, human breast carcinoma tissue, human kidney tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Villin 2 ezrin antibody; CVIL antibody; CVL antibody; Cytovillin 2 antibody; Cytovillin antibody; DKFZp762H157 antibody; Epididymis secretory protein Li 105 antibody; EZR antibody; EZRI_HUMAN antibody; Ezrin antibody; FLJ26216 antibody; HEL S 105 antibody; MGC1584 antibody; p81 antibody; VIL 2 antibody; VIL2 antibody; Villin 2 (ezrin) antibody; Villin 2 antibody; Villin-2 antibody; Villin2 antibody
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.
Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins (2-5). Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.