PRODUCT CODE: ET7109-18

Exportin-5 Recombinant Rabbit Monoclonal Antibody [JE40-91] (ET7109-18)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis of Exportin-5 on 293 cell using anti-Exportin-5 antibody at 1/2,000 dilution.
  • Western blot analysis of Exportin-5 on 293 cell using anti-Exportin-5 antibody at 1/2,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with Exportin-5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Exportin-5 on 293 cell using anti-Exportin-5 antibody at 1/2,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Exportin-5 Recombinant Rabbit Monoclonal Antibody [JE40-91] (ET7109-18)

Immunogen

Recombinant protein within human exportin-5 aa 1-200.

Host

Rabbit

Positive Control

293, rat epididymis tissue, human lung cancer tissue, human thyroid gland tissue, human breast tissue, mouse testis tissue, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE40-91

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

136 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:400

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:100

TARGET

UNIPROT #

PROTEIN NAME

Exportin-5

SYNONYMS

Exp 5 antibody; Exp5 antibody; Exportin 5 antibody; Exportin-5 antibody; Exportin5 antibody; FLJ14239 antibody; FLJ32057 antibody; FLJ45606 antibody; KIAA1291 antibody; OTTHUMP00000016472 antibody; Ran binding protein 21 antibody; Ran-binding protein 21 antibody; Ranbp 21 antibody; Ranbp21 antibody; Xpo 5 antibody; xpo5 antibody; XPO5_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the exportin family.

TISSUE SPECIFICITY

Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

The karyopherin-related nuclear transport factor Exportin 5, also known as Exp5, preferentially recognizes and transports RNAs containing minihelix motifs, structural cis-acting export elements that comprise a double-stranded stem (14 nucleotides) with a base-paired 5' end and a 3-8-nucleotide protruding 3' end. Exportin 5 also mediates protein transport between the nuclear and cytoplasmic compartment. Exportin 5 belongs to a large family of karyopherins and stimulates nuclear export of dsRNA binding proteins eEF1A and tRNA.