ETS1 Recombinant Rabbit Monoclonal Antibody [JM92-32] (ET1705-23)
Catalog# ET1705-23
Rabbit Monoclonal to ETS1
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Daudi cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-23) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ETS1 Recombinant Rabbit Monoclonal Antibody [JM92-32] (ET1705-23)
Immunogen
Synthetic peptide within human ets1 aa 10-59 / 441.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, Daudi cell lysate, human tonsil tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM92-32
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 50 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ETS1
SYNONYMS
Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody;C ets 1 protein antibody;c-ets-1 antibody;ETS 1 antibody;Ets protein antibody;ETS proto-oncogene 1, transcription factor antibody;ETS1 antibody;ETS1 oncogene antibody;ETS1 protein antibody;ETS1_HUMAN antibody;EWSR 2 antibody;EWSR2 antibody;FLJ10768 antibody;Oncogene ETS1 antibody;P54 antibody;Protein C-ets-1 antibody;v ets avian erythroblastosis virus E2 oncogene homolog antibody;v ets avian erythroblastosis virus E2 oncogene homolog 1 antibody;v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody;v ets erythroblastosis virus E26 oncogene homolog 1 antibody;v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
SEQUENCE SIMILARITIES
Belongs to the ETS family.
TISSUE SPECIFICITY
Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.
POST-TRANSLATIONAL MODIFICATION
Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.; Ubiquitinated; which induces proteasomal degradation.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
Protein C-ets-1 is a protein that in humans is encoded by the ETS1 gene. The protein encoded by this gene belongs to the ETS family of transcription factors. There are 28 ETS genes in humans and 27 in mice. They bind the DNA via their winged-helix-turn-helix DNA binding motif known as the Ets domain that specifically recognizes DNA sequences that contain a GGAA/T core element. However, Ets proteins differ significantly in their preference for the sequence flanking the GGAA/T core motif. For instance, the consensus sequence for Ets1 is PuCC/a-GGAA/T-GCPy. On the other hand, many natural Ets1-responsive GGAA/T elements differ from this consensus sequence. The later suggests that several other transcription factors may facilitate Ets1 binding to unfavorable DNA sequences. ChIP-Seq studies have shown that Ets1 can bind both AGGAAG and CGGAAG motifs.
CITATIONS
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Mengdi Yang ,et al
IDO1 inhibitors are synergistic with CXCL10 agonists in inhibiting colon cancer growth
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WB
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Human
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Citation
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