PRODUCT CODE: R1409-1

ERp72 Rabbit Polyclonal Antibody (R1409-1)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of ERp72 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate, untreated <br />
Lane 2: MCF-7 cell lysate, untreated<br />
Lane 3: HepG2 cell lysate, untreated <br />
Lane 4: PANC-1 cell lysate, untreated<br />
Lane 5: PC-12 cell lysate, untreated <br />
Lane 6: Human liver tissue lysate, untreated
  • Western blot analysis of ERp72 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate, untreated <br />
Lane 2: MCF-7 cell lysate, untreated<br />
Lane 3: HepG2 cell lysate, untreated <br />
Lane 4: PANC-1 cell lysate, untreated<br />
Lane 5: PC-12 cell lysate, untreated <br />
Lane 6: Human liver tissue lysate, untreated
  • ICC staining ERp72 in A549 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1409-1) at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining ERp72 in PC-12 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1409-1) at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ERp72 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (R1409-1) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Western blot analysis of ERp72 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate, untreated
Lane 2: MCF-7 cell lysate, untreated
Lane 3: HepG2 cell lysate, untreated
Lane 4: PANC-1 cell lysate, untreated
Lane 5: PC-12 cell lysate, untreated
Lane 6: Human liver tissue lysate, untreated

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ERp72 Rabbit Polyclonal Antibody (R1409-1)

Immunogen

Synthetic peptide of the c-terminal residues of human erp72.

Host

Rabbit

Positive Control

Hela, MCF-7, HepG2, PANC-1, PC-12, human liver tissue, A549, human lung cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

73 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:5,000

  • ICC:1:500

  • IHC-P:1:200

TARGET

UNIPROT #

PROTEIN NAME

ERp72

SYNONYMS

Calcium binding protein intestinal related antibody; Endoplasmic reticulum resident protein 70 antibody; Endoplasmic reticulum resident protein 72 antibody; EPR70 antibody; EPR72 antibody; ER protein 70 antibody; ER protein 72 antibody; ERP 70 antibody; ERp 72 antibody; ERp-72 antibody; ERp70 antibody; ERp72 antibody; PDIA 4 antibody; Pdia4 antibody; PDIA4_HUMAN antibody; PDIR antibody; Protein disulfide isomerase A4 antibody; Protein disulfide isomerase associated 4 antibody; Protein disulfide isomerase family A member 4 antibody; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) antibody; Protein disulfide isomerase related protein antibody; Protein disulfide-isomerase A4 antibody; Protein ERp 72 antibody; Protein ERp72 antibody

SEQUENCE SIMILARITIES

Belongs to the protein disulfide isomerase family.

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. PDI contains four thioredoxin-like domains, two of which retain the canonical CXXC motif. The reduced (that is, dithiol) form of PDI is able to catalyse a reduction of mispaired thiol residues of a particular substrate, acting as an isomerase. Another major function of PDI relates to its activity as a chaperone; i.e., it aids wrongly folded proteins to reach a correctly folded state without the aid of enzymatic disulfide shuffling. PDI helps load antigenic peptides into MHC class I molecules. These molecules (MHC I) are related to the peptide presentation by antigen-presenting cells in the immune response.