ERp72 Mouse Monoclonal Antibody [10-F2] (EM1701-95)
Catalog# EM1701-95
Mouse Monoclonal to ERp72
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Rat
Lysates/proteins at 10 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: 1 minute;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-95) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
ERp72 Mouse Monoclonal Antibody [10-F2] (EM1701-95)
Immunogen
Synthetic peptide within human erp72 aa 596-645 / 645.
Host
Mouse
Positive Control
A431 cell lysates, HeLa, NIH/3T3, rat testis tissue, human lung cancer tissue, human liver tissue, human placenta tissue, mouse brain tissue, SiHa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
10-F2
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:100
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 73 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
ERp72
SYNONYMS
Calcium binding protein intestinal related antibody;Endoplasmic reticulum resident protein 70 antibody;Endoplasmic reticulum resident protein 72 antibody;EPR70 antibody;EPR72 antibody;ER protein 70 antibody;ER protein 72 antibody;ERP 70 antibody;ERp 72 antibody;ERp-72 antibody;ERp70 antibody;ERp72 antibody;PDIA 4 antibody;Pdia4 antibody;PDIA4_HUMAN antibody;PDIR antibody;Protein disulfide isomerase A4 antibody;Protein disulfide isomerase associated 4 antibody;Protein disulfide isomerase family A member 4 antibody;protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) antibody;Protein disulfide isomerase related protein antibody;Protein disulfide-isomerase A4 antibody;Protein ERp 72 antibody;Protein ERp72 antibody
SEQUENCE SIMILARITIES
Belongs to the protein disulfide isomerase family.
SUBCELLULAR LOCATION
Endoplasmic reticulum.
FUNCTION
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly.
CITATIONS
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Sun Yonghua,et al
An intestinal DHA-PA-PG axis promotes digestive organ expansion by mediating usage of maternally-deposited yolk lipids
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IF
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Mouse
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Citation
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Zhang Bei,et al
Unveiling a new strategy for PDIA1 inhibition: Integration of activity-based probes profiling and targeted degradation
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WB
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Human
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Citation
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