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PRODUCT CODE: EM1701-95

ERp72 Mouse Monoclonal Antibody [10-F2] (EM1701-95)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of ERp72 on A431 cell lysate using anti-ERp72 antibody at 1/500 dilution.
  • Western blot analysis of ERp72 on A431 cell lysate using anti-ERp72 antibody at 1/500 dilution.
  • ICC staining ERp72 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ERp72 (green) in SKOV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SiHa cells with ERp72 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of ERp72 on A431 cell lysate using anti-ERp72 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

ERp72 Mouse Monoclonal Antibody [10-F2] (EM1701-95)

Immunogen

Synthetic peptide within human erp72 aa 596-645 / 645.

Host

Mouse

Positive Control

A431, HepG2, SiHa, SKOV-3, human lung cancer tissue, human liver tissue, rat testis tissue, human placenta tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

10-F2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

ProG purified.

MOLECULAR WEIGHT

73 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P13667

PROTEIN NAME

ERp72

SYNONYMS

Calcium binding protein intestinal related antibody; Endoplasmic reticulum resident protein 70 antibody; Endoplasmic reticulum resident protein 72 antibody; EPR70 antibody; EPR72 antibody; ER protein 70 antibody; ER protein 72 antibody; ERP 70 antibody; ERp 72 antibody; ERp-72 antibody; ERp70 antibody; ERp72 antibody; PDIA 4 antibody; Pdia4 antibody; PDIA4_HUMAN antibody; PDIR antibody; Protein disulfide isomerase A4 antibody; Protein disulfide isomerase associated 4 antibody; Protein disulfide isomerase family A member 4 antibody; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) antibody; Protein disulfide isomerase related protein antibody; Protein disulfide-isomerase A4 antibody; Protein ERp 72 antibody; Protein ERp72 antibody

SEQUENCE SIMILARITIES

Belongs to the protein disulfide isomerase family.

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly.