PDIA3 / ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44] (ET1703-68)
Catalog# ET1703-68
Rabbit Monoclonal to ERp57
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WB
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IP
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IHC-P
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Human
Lane 1: HepG2 cell lysate
Lane 2: LNCaP cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-68) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PDIA3 / ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44] (ET1703-68)
Immunogen
Synthetic peptide within human pdia3 aa 175-208 / 505.
Host
Rabbit
Positive Control
HepG2 cell lysate, LNCaP cell lysate, human kidney tissue, human pancreas tissue, human uterus tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM11-44
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:200
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IP
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1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 57 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ERp57
SYNONYMS
58 kDa glucose regulated protein antibody;58 kDa glucose-regulated protein antibody;58 kDa microsomal protein antibody;Disulfide isomerase ER 60 antibody;Disulfide isomerase ER-60 antibody;Endoplasmic reticulum resident protein 57 antibody;Endoplasmic reticulum resident protein 60 antibody;ER p57 antibody;ER protein 57 antibody;ER protein 60 antibody;ERp 57 antibody;ERp57 antibody;ERp60 antibody;ERp61 antibody;Glucose Regulated Protein 58 Kd antibody;GRP 57 antibody;GRP 58 antibody;GRP57 antibody;HsT17083 antibody;p58 antibody;PDIA 3 antibody;PDIA3 antibody;PDIA3_HUMAN antibody;Phospholipase C alpha antibody;PI PLC antibody;Protein disulfide isomerase A3 antibody;Protein disulfide isomerase family A member 3 antibody;Protein disulfide-isomerase A3 antibody
SEQUENCE SIMILARITIES
Belongs to the protein disulfide isomerase family.
TISSUE SPECIFICITY
Detected in the flagellum and head region of spermatozoa (at protein level).
SUBCELLULAR LOCATION
Endoplasmic reticulum lumen. Melanosome.
FUNCTION
Mammals defend themselves against intracellular pathogens through presentation of cytoplasmically-derived short pathogenic peptides to the cell surface of cytotoxic T lymphocytes, which subsequently leads to cytotoxic events with respect to the affected cell. Antigen presentation is mediated by major histocompatibility complex (MHC) class I molecules, which bind and coordinate short pathogenic peptides. MHC class I molecules assemble in the endoplasmic reticulum with chaperones before binding to the transporter associated with antigen processing (TAP). ERp57, also designated GRP57, GRP58, ERp60 and ERp61, is a component of the MHC class I pathway that appears to interact with MHC class I molecules before they associate with TAP. The human ERp57 gene maps to chromosome 15q15 and encodes a 505 amino acid protein. ERp57 has two Trp-Cys-Gly-His-Cys-Lys motifs completely conserved among the mammals. ERp57 may act as a protease, a protein disulfide isomerase, a phospholipase or a combination of these.
CITATIONS
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Zhang Bei,et al
Unveiling a new strategy for PDIA1 inhibition: Integration of activity-based probes profiling and targeted degradation
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WB
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Human
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Citation
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