ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44] (ET1703-68)

Western blot analysis of ERp57 on different cells lysates using anti-ERp57 antibody at 1/500 dilution.<br />
Positive control: <br />
Line 1: Hela <br />
Line 2: HepG2 <br />
Line 3:293T

Western blot analysis of ERp57 on different cells lysates using anti-ERp57 antibody at 1/500 dilution.
Positive control:
Line 1: Hela
Line 2: HepG2
Line 3:293T

Applications

WB
IP
IHC-P

REACTIVITY

Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44] (ET1703-68)

Immunogen

Synthetic peptide within human erp57 aa 175-208 / 505.

Host

Rabbit

Positive Control

HepG2, 293T, Hela, human pancreas tissue, human kidney tissue, human uterus tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-44

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

WB
1:500-1:1,000

IHC-P
1:50-1:200

IP
1:10-1:50

TARGET

UNIPROT #

P30101

PROTEIN NAME

ERp57

SYNONYMS

58 kDa glucose regulated protein antibody; 58 kDa glucose-regulated protein antibody; 58 kDa microsomal protein antibody; Disulfide isomerase ER 60 antibody; Disulfide isomerase ER-60 antibody; Endoplasmic reticulum resident protein 57 antibody; Endoplasmic reticulum resident protein 60 antibody; ER p57 antibody; ER protein 57 antibody; ER protein 60 antibody; ERp 57 antibody; ERp57 antibody; ERp60 antibody; ERp61 antibody; Glucose Regulated Protein 58 Kd antibody; GRP 57 antibody; GRP 58 antibody; GRP57 antibody; HsT17083 antibody; p58 antibody; PDIA 3 antibody; PDIA3 antibody; PDIA3_HUMAN antibody; Phospholipase C alpha antibody; PI PLC antibody; Protein disulfide isomerase A3 antibody; Protein disulfide isomerase family A member 3 antibody; Protein disulfide-isomerase A3 antibody

SEQUENCE SIMILARITIES

Belongs to the protein disulfide isomerase family.

TISSUE SPECIFICITY

Detected in the flagellum and head region of spermatozoa (at protein level).

SUBCELLULAR LOCATION

Endoplasmic reticulum lumen. Melanosome.

FUNCTION

Mammals defend themselves against intracellular pathogens through presentation of cytoplasmically-derived short pathogenic peptides to the cell surface of cytotoxic T lymphocytes, which subsequently leads to cytotoxic events with respect to the affected cell. Antigen presentation is mediated by major histocompatibility complex (MHC) class I molecules, which bind and coordinate short pathogenic peptides. MHC class I molecules assemble in the endoplasmic reticulum with chaperones before binding to the transporter associated with antigen processing (TAP). ERp57, also designated GRP57, GRP58, ERp60 and ERp61, is a component of the MHC class I pathway that appears to interact with MHC class I molecules before they associate with TAP. The human ERp57 gene maps to chromosome 15q15 and encodes a 505 amino acid protein. ERp57 has two Trp-Cys-Gly-His-Cys-Lys motifs completely conserved among the mammals. ERp57 may act as a protease, a protein disulfide isomerase, a phospholipase or a combination of these.