ERN1 Rabbit Polyclonal Antibody (ER1902-90)
Catalog# ER1902-90
Rabbit Polyclonal to ERN1
Tested for
-
WB
-
IHC-P
Reacts with
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Human
-
Mouse
-
Rat
Western blot analysis of IRE1 on different lysates with Rabbit anti-IRE1 antibody (ER1902-90) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-90) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-90) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.