ERK1 Rabbit Polyclonal Antibody (ER131011)

Western blot analysis of ERK1 on different cell lysates using anti- ERK1 antibody at 1/1000 dilution.<br />
Positive control:<br />
Lane 1: Hela<br />
Lane 2: SW480<br />
Lane 3: HCT116<br />
Lane 4: HepG2<br />
Lane 5: PC12

Western blot analysis of ERK1 on different cell lysates using anti- ERK1 antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: SW480
Lane 3: HCT116
Lane 4: HepG2
Lane 5: PC12

Applications

WB
ICC
IHC-P

REACTIVITY

Human
Mouse
Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ERK1 Rabbit Polyclonal Antibody (ER131011)

Immunogen

Synthetic peptide within human erk1 aa 306-360.

Host

Rabbit

Positive Control

Hela, SW480, HCT116, HepG2, PC12, LOVO, human colon carcinoma tissue, human breast carcinoma tissue, mouse large intestine tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

44 kDa

Isotype

IgG

APPLICATION DILUTION

WB
1:500-1:1,000

ICC
1:200

IHC-P
1:200

TARGET

UNIPROT #

P27361

PROTEIN NAME

ERK1

SYNONYMS

ERK 1 antibody; ERK antibody; ERK-1 antibody; ERK1 antibody; ERT 2 antibody; ERT2 antibody; Extracellular Signal Regulated Kinase 1 antibody; Extracellular signal related kinase 1 antibody; Extracellular signal-regulated kinase 1 antibody; HGNC6877 antibody; HS44KDAP antibody; HUMKER1A antibody; Insulin Stimulated MAP2 Kinase antibody; Insulin-stimulated MAP2 kinase antibody; MAP kinase 1 antibody; MAP kinase 3 antibody; MAP Kinase antibody; MAP kinase isoform p44 antibody; MAPK 1 antibody; MAPK 3 antibody; MAPK antibody; MAPK1 antibody; Mapk3 antibody; MGC20180 antibody; Microtubule Associated Protein 2 Kinase antibody; Microtubule-associated protein 2 kinase antibody; Mitogen Activated Protein Kinase 3 antibody; Mitogen-activated protein kinase 1 antibody; Mitogen-activated protein kinase 3 antibody; MK03_HUMAN antibody; OTTHUMP00000174538 antibody; OTTHUMP00000174541 antibody; p44 ERK1 antibody; p44 MAPK antibody; p44-ERK1 antibody; p44-MAPK antibody; P44ERK1 antibody; P44MAPK antibody; PRKM 3 antibody; PRKM3 antibody; Protein Kinase Mitogen Activated 3 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.

SUBCELLULAR LOCATION

Cytoplasm, nucleus

FUNCTION

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines , and research investigators consider it an important target in the diagnosis and treatment of cancer . Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway.