ERK1/2 Recombinant Rabbit Monoclonal Antibody [SA43-03] (ET1601-29)
Catalog# ET1601-29
Rabbit Monoclonal to ERK1/2
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WB
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IF-Cell
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IF-Tissue
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IP
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FC
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IHC-P
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IHC-Fr
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Human
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Mouse
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Rat
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Zebrafish
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HepG2 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 43/41 kDa
Observed band size: 43/41 kDa
Exposure time: 19 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-29) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ERK1/2 Recombinant Rabbit Monoclonal Antibody [SA43-03] (ET1601-29)
Immunogen
Recombinant protein within human erk2 aa 180-379.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, Jurkat, human breast carcinoma tissue, mouse esophagus tissue, mouse stomach tissue, Hela, MCF-7, NIH/3T3, A549.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA43-03
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:2,000
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IHC-P
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1:20,000
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IHC-Fr
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1:50
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IF-Tissue
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1:50
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 43/41 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ERK1/2
SYNONYMS
ERK 1 antibody;ERK 2 antibody;ERK-2 antibody;ERK1 antibody;erk1/2 antibody;ERK2 antibody;ERT1 antibody;ERT2 antibody;Extracellular signal regulated kinase 1 antibody;Extracellular signal-regulated kinase 2 antibody;MAP kinase 1 antibody;MAP kinase 2 antibody;MAP kinase isoform p42 antibody;MAP kinase isoform p44 antibody;MAPK 1 antibody;MAPK 2 antibody;MAPK 3 antibody;Mapk1 antibody;MAPK2 antibody;MAPK3 antibody;Mitogen-activated protein kinase 1 antibody;Mitogen-activated protein kinase 2 antibody;MK01_HUMAN antibody;p38 antibody;p40 antibody;p41 antibody;p42-MAPK antibody;PRKM 2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
The first mitogen-activated protein kinase to be discovered was ERK1 (MAPK3) in mammals. Since ERK1 and its close relative ERK2 (MAPK1) are both involved in growth factor signaling, the family was termed "mitogen-activated". With the discovery of other members, even from distant organisms (e.g. plants), it has become increasingly clear that the name is a misnomer, since most MAPKs are actually involved in the response to potentially harmful, abiotic stress stimuli (hyperosmosis, oxidative stress, DNA damage, low osmolarity, infection, etc.). Because plants cannot "flee" from stress, terrestrial plants have the highest number of MAPK genes per organism ever found[citation needed]. Thus the role of mammalian ERK1/2 kinases as regulators of cell proliferation is not a generic, but a highly specialized function.
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