PRODUCT CODE: ER1802-98

ERGI3 Rabbit Polyclonal Antibody (ER1802-98)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ERGI3 on different lysates using anti-ERGI3 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: SiHa  <br />
      Lane 2: Human placenta<br />
 Lane 3: Rat bone marrow
  • Western blot analysis of ERGI3 on different lysates using anti-ERGI3 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: SiHa  <br />
      Lane 2: Human placenta<br />
 Lane 3: Rat bone marrow
  • ICC staining ERGI3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ERGI3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with ERGI3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of ERGI3 on different lysates using anti-ERGI3 antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa
Lane 2: Human placenta
Lane 3: Rat bone marrow

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ERGI3 Rabbit Polyclonal Antibody (ER1802-98)

Immunogen

Synthetic peptide within c-terminal human ergi3.

Host

Rabbit

Positive Control

Siha, human placenta tissue, rat bone marrow tissue, LOVO, human colon cancer tissue, human uterus tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ERGI3

SYNONYMS

2310015B14Rik antibody; AV318804 antibody; C20orf47 antibody; CGI 54 antibody; D2Ucla1 antibody; dJ477O4.2 antibody; DKFZp547A2190 antibody; Endoplasmic reticulum Golgi intermediate compartment protein 3 antibody; endoplasmic reticulum localized protein ERp43 antibody; Endoplasmic reticulum-Golgi intermediate compartment protein 3 antibody; ERGI3_HUMAN antibody; ERGIC and golgi 3 antibody; ergic3 antibody; ERV46 antibody; NY BR 84 antibody; PRO0989 antibody; RP23-220D12.2 antibody; RP3-477O4.1 antibody; SDBCAG84 antibody; Serologically defined breast cancer antigen 84 antibody; Serologically defined breast cancer antigen NY BR 84 antibody; Serologically defined breast cancer antigen NY-BR-84 antibody

SEQUENCE SIMILARITIES

Belongs to the ERGIC family.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Golgi apparatus.

FUNCTION

Belongs to the ERGIC family. The ERGIC lies between the rough endoplasmic reticulum and Golgi on the secretory pathway. The vesicular-tubular cluster (VTC), also referred to as the ER-Golgi intermediate compartment (or ERGIC), is an organelle in eukaryotic cells. This compartment mediates trafficking between the endoplasmic reticulum and Golgi complex, facilitating the sorting of cargo. ERGI3 possible role in transport between endoplasmic reticulum and Golgi.